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A fast analysis system for forensic DNA reference samples

Hedman, Johannes LU ; Albinsson, Linda; Ansell, Carina; Tapper, Helene; Hansson, Oskar; Holgersson, Stig and Ansell, Ricky (2008) In Forensic Science International: Genetics 2(3). p.184-189
Abstract
On January 1st, 2006, the Swedish legislation on obtaining DNA reference samples from Suspects and the recording of DNA profiles in databases was changed. As a result the number of samples analysed at the Swedish National Laboratory of Forensic Science (SKL) increased from about 4500 in 2005 to more than 25,000 in 2006. To meet this challenge, SKL launched a flew analysis system to create an unbroken chain, from sampling to incorporation of a profile in the national DNA database and subsequent automatic generation of digitally signed hit reports. The system integrates logistics, digital data transfer, new functions in LIMS (ForumDNA Version 4, Ida Infront AB) and laboratory automation. Buccal swab samples are secured on a FTA (R) card... (More)
On January 1st, 2006, the Swedish legislation on obtaining DNA reference samples from Suspects and the recording of DNA profiles in databases was changed. As a result the number of samples analysed at the Swedish National Laboratory of Forensic Science (SKL) increased from about 4500 in 2005 to more than 25,000 in 2006. To meet this challenge, SKL launched a flew analysis system to create an unbroken chain, from sampling to incorporation of a profile in the national DNA database and subsequent automatic generation of digitally signed hit reports. The system integrates logistics, digital data transfer, new functions in LIMS (ForumDNA Version 4, Ida Infront AB) and laboratory automation. Buccal swab samples are secured on a FTA (R) card attached to an identity form, which is barcoded with a unique sample ID. After sampling, the police officer sends a digital request to SKL. The sample is automatically registered in LIMS and processed on delivery. The resulting DNA profiles are automatically classified according to quality using a custom-made expert system. Building the evaluation around mathematical rules makes it reproducible, standardised and minimises manual work and clerk errors. All samples are run in duplicate and the two profiles are compared within LIMS before incorporation in the database. In the first year of operation, the median time for completion of an analysis was 3 days, measured from delivery of the sample to incorporation of the profile in the national DNA database. In spite of the dramatic increase in the number of reference samples there was no backlog. (C) 2008 Elsevier Ireland Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA database, Forensic DNA analysis, Reference sample, Automation, LIMS
in
Forensic Science International: Genetics
volume
2
issue
3
pages
184 - 189
publisher
Elsevier
external identifiers
  • wos:000261500400004
  • scopus:43049083993
ISSN
1878-0326
DOI
10.1016/j.fsigen.2007.12.011
language
English
LU publication?
yes
id
54ddf3d9-53d2-4d51-9e75-2af0ca0a783b (old id 1305635)
date added to LUP
2009-03-23 12:03:30
date last changed
2017-01-01 04:50:39
@article{54ddf3d9-53d2-4d51-9e75-2af0ca0a783b,
  abstract     = {On January 1st, 2006, the Swedish legislation on obtaining DNA reference samples from Suspects and the recording of DNA profiles in databases was changed. As a result the number of samples analysed at the Swedish National Laboratory of Forensic Science (SKL) increased from about 4500 in 2005 to more than 25,000 in 2006. To meet this challenge, SKL launched a flew analysis system to create an unbroken chain, from sampling to incorporation of a profile in the national DNA database and subsequent automatic generation of digitally signed hit reports. The system integrates logistics, digital data transfer, new functions in LIMS (ForumDNA Version 4, Ida Infront AB) and laboratory automation. Buccal swab samples are secured on a FTA (R) card attached to an identity form, which is barcoded with a unique sample ID. After sampling, the police officer sends a digital request to SKL. The sample is automatically registered in LIMS and processed on delivery. The resulting DNA profiles are automatically classified according to quality using a custom-made expert system. Building the evaluation around mathematical rules makes it reproducible, standardised and minimises manual work and clerk errors. All samples are run in duplicate and the two profiles are compared within LIMS before incorporation in the database. In the first year of operation, the median time for completion of an analysis was 3 days, measured from delivery of the sample to incorporation of the profile in the national DNA database. In spite of the dramatic increase in the number of reference samples there was no backlog. (C) 2008 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Hedman, Johannes and Albinsson, Linda and Ansell, Carina and Tapper, Helene and Hansson, Oskar and Holgersson, Stig and Ansell, Ricky},
  issn         = {1878-0326},
  keyword      = {DNA database,Forensic DNA analysis,Reference sample,Automation,LIMS},
  language     = {eng},
  number       = {3},
  pages        = {184--189},
  publisher    = {Elsevier},
  series       = {Forensic Science International: Genetics},
  title        = {A fast analysis system for forensic DNA reference samples},
  url          = {http://dx.doi.org/10.1016/j.fsigen.2007.12.011},
  volume       = {2},
  year         = {2008},
}