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Cystometric findings in mice lacking muscarinic M2 or M3 receptors

Igawa, Y; Zhang, X; Nishizawa, O; Umeda, M; Iwata, A; Taketo, M M; Manabe, T; Matsui, M and Andersson, Karl-Erik LU (2004) In Journal of Urology 172(6, Part 1 of 2). p.2460-2464
Abstract
Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in... (More)
Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in WT and M-2 KO mice but it had no effect on any cystometric parameters in M-3 KO mice. Conclusions: The current results confirm that M-3 receptor is the principal muscarinic receptor subtype responsible for bladder contraction and the role of M-2 receptors is of minor importance. Functional impairments found in M-3 KO mice were milder than those elicited by acute blockade of muscarinic receptors by atropine in WT mice, suggesting that noncholinergic mechanisms can compensate for a chronic loss of M-3 receptors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
receptors, bladder, purinergic, muscarinic, atropine, mice
in
Journal of Urology
volume
172
issue
6, Part 1 of 2
pages
2460 - 2464
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000225194900081
  • pmid:15538291
  • scopus:8644286767
ISSN
1527-3792
DOI
10.1097/01.ju.0000138054.77785.4a
language
English
LU publication?
yes
id
29b596f7-d8dd-43a4-a199-a06fe3ac2592 (old id 130886)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15538291&dopt=Abstract
date added to LUP
2007-07-04 14:56:27
date last changed
2017-08-20 04:21:56
@article{29b596f7-d8dd-43a4-a199-a06fe3ac2592,
  abstract     = {Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in WT and M-2 KO mice but it had no effect on any cystometric parameters in M-3 KO mice. Conclusions: The current results confirm that M-3 receptor is the principal muscarinic receptor subtype responsible for bladder contraction and the role of M-2 receptors is of minor importance. Functional impairments found in M-3 KO mice were milder than those elicited by acute blockade of muscarinic receptors by atropine in WT mice, suggesting that noncholinergic mechanisms can compensate for a chronic loss of M-3 receptors.},
  author       = {Igawa, Y and Zhang, X and Nishizawa, O and Umeda, M and Iwata, A and Taketo, M M and Manabe, T and Matsui, M and Andersson, Karl-Erik},
  issn         = {1527-3792},
  keyword      = {receptors,bladder,purinergic,muscarinic,atropine,mice},
  language     = {eng},
  number       = {6, Part 1 of 2},
  pages        = {2460--2464},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Cystometric findings in mice lacking muscarinic M2 or M3 receptors},
  url          = {http://dx.doi.org/10.1097/01.ju.0000138054.77785.4a},
  volume       = {172},
  year         = {2004},
}