Cystometric findings in mice lacking muscarinic M2 or M3 receptors
Igawa, Y ; Zhang, X ; Nishizawa, O ; Umeda, M ; Iwata, A ; Taketo, M M ; Manabe, T ; Matsui, M and Andersson, Karl-Erik LU
(2004)
In Journal of Urology
172(6, Part 1 of 2).
p.2460-2464
- Abstract
- Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in... (More)
- Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in WT and M-2 KO mice but it had no effect on any cystometric parameters in M-3 KO mice. Conclusions: The current results confirm that M-3 receptor is the principal muscarinic receptor subtype responsible for bladder contraction and the role of M-2 receptors is of minor importance. Functional impairments found in M-3 KO mice were milder than those elicited by acute blockade of muscarinic receptors by atropine in WT mice, suggesting that noncholinergic mechanisms can compensate for a chronic loss of M-3 receptors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/130886
- author
- Igawa, Y
; Zhang, X
; Nishizawa, O
; Umeda, M
; Iwata, A
; Taketo, M M
; Manabe, T
; Matsui, M
and Andersson, Karl-Erik
LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- receptors, bladder, purinergic, muscarinic, atropine, mice
- in
- Journal of Urology
- volume
- 172
- issue
- 6, Part 1 of 2
- pages
- 2460 - 2464
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000225194900081
- pmid:15538291
- scopus:8644286767
- ISSN
- 1527-3792
- DOI
- 10.1097/01.ju.0000138054.77785.4a
- language
- English
- LU publication?
- yes
- id
- 29b596f7-d8dd-43a4-a199-a06fe3ac2592 (old id 130886)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15538291&dopt=Abstract
- date added to LUP
- 2016-04-01 16:05:45
- date last changed
- 2022-03-07 03:33:42
@article{29b596f7-d8dd-43a4-a199-a06fe3ac2592,
abstract = {{Purpose: The physiological importance of muscarinic M-3 and M-2 receptors for bladder function was investigated in vivo using mice lacking M-3 or M-2 receptors and littermate WT controls. Materials and Methods: Unanesthetized mice of each sex underwent continuous cystometry before and after administration of atropine (1 mg/kg(-1)). Results: Male M-3 knockout (KO) mice had longer voiding intervals, and larger micturition volumes and bladder capacity than M-2 KO or WT males. There was no significant difference in any cystometric parameters between male M-2 KO and WT mice. In females M-3 KO and M-2 KO mice had longer voiding intervals and larger micturition volumes than WT animals. Atropine had marked inhibitory effects on voiding efficacy in WT and M-2 KO mice but it had no effect on any cystometric parameters in M-3 KO mice. Conclusions: The current results confirm that M-3 receptor is the principal muscarinic receptor subtype responsible for bladder contraction and the role of M-2 receptors is of minor importance. Functional impairments found in M-3 KO mice were milder than those elicited by acute blockade of muscarinic receptors by atropine in WT mice, suggesting that noncholinergic mechanisms can compensate for a chronic loss of M-3 receptors.}},
author = {{Igawa, Y and Zhang, X and Nishizawa, O and Umeda, M and Iwata, A and Taketo, M M and Manabe, T and Matsui, M and Andersson, Karl-Erik}},
issn = {{1527-3792}},
keywords = {{receptors; bladder; purinergic; muscarinic; atropine; mice}},
language = {{eng}},
number = {{6, Part 1 of 2}},
pages = {{2460--2464}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Journal of Urology}},
title = {{Cystometric findings in mice lacking muscarinic M2 or M3 receptors}},
url = {{http://dx.doi.org/10.1097/01.ju.0000138054.77785.4a}},
doi = {{10.1097/01.ju.0000138054.77785.4a}},
volume = {{172}},
year = {{2004}},
}