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Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters

Bjork, Per; Thomsson, Daniel LU ; Mirzov, Oleg LU ; Wigenius, Jens; Inganas, Olle and Scheblykin, Ivan LU (2009) In Small 5(1). p.96-103
Abstract
The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced... (More)
The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
single-molecule, fluorescence, DNA, aggregation, conjugated polymers, spectroscopy
in
Small
volume
5
issue
1
pages
96 - 103
publisher
John Wiley & Sons
external identifiers
  • wos:000262895300015
  • scopus:58149459134
ISSN
1613-6829
DOI
10.1002/smll.200800855
language
English
LU publication?
yes
id
b4c49749-ce2f-4771-ab8f-10c14f0b15f7 (old id 1308868)
date added to LUP
2009-03-23 16:02:23
date last changed
2017-04-02 03:36:31
@article{b4c49749-ce2f-4771-ab8f-10c14f0b15f7,
  abstract     = {The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.},
  author       = {Bjork, Per and Thomsson, Daniel and Mirzov, Oleg and Wigenius, Jens and Inganas, Olle and Scheblykin, Ivan},
  issn         = {1613-6829},
  keyword      = {single-molecule,fluorescence,DNA,aggregation,conjugated polymers,spectroscopy},
  language     = {eng},
  number       = {1},
  pages        = {96--103},
  publisher    = {John Wiley & Sons},
  series       = {Small},
  title        = {Oligothiophene Assemblies Defined by DNA Interaction: From Single Chains to Disordered Clusters},
  url          = {http://dx.doi.org/10.1002/smll.200800855},
  volume       = {5},
  year         = {2009},
}