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Macroporous elastic polyacrylamide monolith columns: processing under compression and scale-up

Plieva, Fatima LU ; De Seta, Elena; Galaev, Igor LU and Mattiasson, Bo LU (2009) In Separation and Purification Technology 65(1). p.110-116
Abstract
Macroporous gel (MG) monoliths are novel stationary media with a combination of unique properties such as continuous system of interconnected macropores and high elasticity. Due to the large (1-100 mu m) and highly interconnected pores, the polyacrylamide (pAAm) MG monolith columns have low backpressure and can be processed at high flow rates. The highly open macroporous structure with elastic pore walls in pAAm MG monoliths allow for processing under compression and construction of scaled MG monolith columns (when the MG monoliths of the same diameter are placed on top of each other, i.e. composed column). High elasticity of pore walls in the pAAm MGs allows for significant uniaxial compression of monoliths without their breaking and... (More)
Macroporous gel (MG) monoliths are novel stationary media with a combination of unique properties such as continuous system of interconnected macropores and high elasticity. Due to the large (1-100 mu m) and highly interconnected pores, the polyacrylamide (pAAm) MG monolith columns have low backpressure and can be processed at high flow rates. The highly open macroporous structure with elastic pore walls in pAAm MG monoliths allow for processing under compression and construction of scaled MG monolith columns (when the MG monoliths of the same diameter are placed on top of each other, i.e. composed column). High elasticity of pore walls in the pAAm MGs allows for significant uniaxial compression of monoliths without their breaking and losing column efficiency. A novel approach for elution of bound proteins through the compressed MG monoliths is presented. More than 80% of the bound model protein lysozyme was eluted from the compressed iminodiacetate (IDA) pAAm MGs monoliths (50% compression of initial monolith height) with up to double protein concentration in the peak elution fractions compared to elution through non-compressed monolith. (C) 2008 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Compressed monolith, Composed monolith column, Scale-up, Elasticity, Polyacrylamide, Macroporous gel, Cryogel
in
Separation and Purification Technology
volume
65
issue
1
pages
110 - 116
publisher
Elsevier
external identifiers
  • wos:000263021000016
  • scopus:58149277042
ISSN
1873-3794
DOI
10.1016/j.seppur.2008.03.026
language
English
LU publication?
yes
id
5fb2d8ec-aa15-4568-9c25-3f53714b366e (old id 1308891)
date added to LUP
2009-03-23 16:10:08
date last changed
2017-10-22 03:54:27
@article{5fb2d8ec-aa15-4568-9c25-3f53714b366e,
  abstract     = {Macroporous gel (MG) monoliths are novel stationary media with a combination of unique properties such as continuous system of interconnected macropores and high elasticity. Due to the large (1-100 mu m) and highly interconnected pores, the polyacrylamide (pAAm) MG monolith columns have low backpressure and can be processed at high flow rates. The highly open macroporous structure with elastic pore walls in pAAm MG monoliths allow for processing under compression and construction of scaled MG monolith columns (when the MG monoliths of the same diameter are placed on top of each other, i.e. composed column). High elasticity of pore walls in the pAAm MGs allows for significant uniaxial compression of monoliths without their breaking and losing column efficiency. A novel approach for elution of bound proteins through the compressed MG monoliths is presented. More than 80% of the bound model protein lysozyme was eluted from the compressed iminodiacetate (IDA) pAAm MGs monoliths (50% compression of initial monolith height) with up to double protein concentration in the peak elution fractions compared to elution through non-compressed monolith. (C) 2008 Elsevier B.V. All rights reserved.},
  author       = {Plieva, Fatima and De Seta, Elena and Galaev, Igor and Mattiasson, Bo},
  issn         = {1873-3794},
  keyword      = {Compressed monolith,Composed monolith column,Scale-up,Elasticity,Polyacrylamide,Macroporous gel,Cryogel},
  language     = {eng},
  number       = {1},
  pages        = {110--116},
  publisher    = {Elsevier},
  series       = {Separation and Purification Technology},
  title        = {Macroporous elastic polyacrylamide monolith columns: processing under compression and scale-up},
  url          = {http://dx.doi.org/10.1016/j.seppur.2008.03.026},
  volume       = {65},
  year         = {2009},
}