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High turnover of fungal hyphae in incubation experiments

de Vries, Franciska T.; Bååth, Erland LU ; Kuyper, Thom W. and Bloem, Jaap (2009) In FEMS Microbiology Ecology 67(3). p.389-396
Abstract
Soil biological studies are often conducted on sieved soils without the presence of plants. However, soil fungi build delicate mycelial networks, often symbiotically associated with plant roots (mycorrhizal fungi). We hypothesized that as a result of sieving and incubating without plants, the total fungal biomass decreases. To test this, we conducted three incubation experiments. We expected total and arbuscular mycorrhizal (AM) fungal biomass to be higher in less fertilized soils than in fertilized soils, and thus to decrease more during incubation. Indeed, we found that fungal biomass decreased rapidly in the less fertilized soils. A shift towards thicker hyphae occurred, and the fraction of septate hyphae increased. However, analyses of... (More)
Soil biological studies are often conducted on sieved soils without the presence of plants. However, soil fungi build delicate mycelial networks, often symbiotically associated with plant roots (mycorrhizal fungi). We hypothesized that as a result of sieving and incubating without plants, the total fungal biomass decreases. To test this, we conducted three incubation experiments. We expected total and arbuscular mycorrhizal (AM) fungal biomass to be higher in less fertilized soils than in fertilized soils, and thus to decrease more during incubation. Indeed, we found that fungal biomass decreased rapidly in the less fertilized soils. A shift towards thicker hyphae occurred, and the fraction of septate hyphae increased. However, analyses of phospholipid fatty acids (PLFAs) and neutral lipid fatty acids could not clarify which fungal groups were decreasing. We propose that in our soils, there was a fraction of fungal biomass that was sensitive to fertilization and disturbance (sieving, followed by incubation without plants) with a very high turnover (possibly composed of fine hyphae of AM and saprotrophic fungi), and a fraction that was much less vulnerable with a low turnover (composed of saprotrophic fungi and runner hyphae of AMF). Furthermore, PLFAs might not be as sensitive in detecting changes in fungal biomass as previously thought. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
neutral lipid fatty acid (NLFA), microscopic counting, fatty acid (PLFA), phospholipid, saprotrophic fungi, arbuscular mycorrhizal fungi (AMF)
in
FEMS Microbiology Ecology
volume
67
issue
3
pages
389 - 396
publisher
Elsevier
external identifiers
  • wos:000262952300005
  • scopus:59249108064
ISSN
1574-6941
DOI
10.1111/j.1574-6941.2008.00643.x
language
English
LU publication?
yes
id
d9a89a33-76b0-4757-8548-37a9a11c24ff (old id 1311506)
date added to LUP
2009-03-17 10:18:11
date last changed
2017-07-09 03:49:47
@article{d9a89a33-76b0-4757-8548-37a9a11c24ff,
  abstract     = {Soil biological studies are often conducted on sieved soils without the presence of plants. However, soil fungi build delicate mycelial networks, often symbiotically associated with plant roots (mycorrhizal fungi). We hypothesized that as a result of sieving and incubating without plants, the total fungal biomass decreases. To test this, we conducted three incubation experiments. We expected total and arbuscular mycorrhizal (AM) fungal biomass to be higher in less fertilized soils than in fertilized soils, and thus to decrease more during incubation. Indeed, we found that fungal biomass decreased rapidly in the less fertilized soils. A shift towards thicker hyphae occurred, and the fraction of septate hyphae increased. However, analyses of phospholipid fatty acids (PLFAs) and neutral lipid fatty acids could not clarify which fungal groups were decreasing. We propose that in our soils, there was a fraction of fungal biomass that was sensitive to fertilization and disturbance (sieving, followed by incubation without plants) with a very high turnover (possibly composed of fine hyphae of AM and saprotrophic fungi), and a fraction that was much less vulnerable with a low turnover (composed of saprotrophic fungi and runner hyphae of AMF). Furthermore, PLFAs might not be as sensitive in detecting changes in fungal biomass as previously thought.},
  author       = {de Vries, Franciska T. and Bååth, Erland and Kuyper, Thom W. and Bloem, Jaap},
  issn         = {1574-6941},
  keyword      = {neutral lipid fatty acid (NLFA),microscopic counting,fatty acid (PLFA),phospholipid,saprotrophic fungi,arbuscular mycorrhizal fungi (AMF)},
  language     = {eng},
  number       = {3},
  pages        = {389--396},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {High turnover of fungal hyphae in incubation experiments},
  url          = {http://dx.doi.org/10.1111/j.1574-6941.2008.00643.x},
  volume       = {67},
  year         = {2009},
}