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Protochlorophyllide a: A Comprehensive Photophysical Picture

Dietzek, Benjamin; Tschierlei, Stefanie; Hermann, Gudrun; Yartsev, Arkady LU ; Pascher, Torbjörn LU ; Sundström, Villy LU ; Schmitt, Michael and Popp, Juergen (2009) In ChemPhysChem 10(1). p.144-150
Abstract
The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed... (More)
The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transient absorption, porphyrins, enzyme catalysis, photophysics, spectroscopy, UV/Vis spectroscopy
in
ChemPhysChem
volume
10
issue
1
pages
144 - 150
publisher
John Wiley & Sons
external identifiers
  • wos:000262870500020
  • scopus:58149506584
ISSN
1439-7641
DOI
10.1002/cphc.200800536
language
English
LU publication?
yes
id
676fbd97-4d4a-411a-8352-447a194ffffa (old id 1311577)
date added to LUP
2009-03-16 15:47:42
date last changed
2017-11-19 03:25:10
@article{676fbd97-4d4a-411a-8352-447a194ffffa,
  abstract     = {The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed.},
  author       = {Dietzek, Benjamin and Tschierlei, Stefanie and Hermann, Gudrun and Yartsev, Arkady and Pascher, Torbjörn and Sundström, Villy and Schmitt, Michael and Popp, Juergen},
  issn         = {1439-7641},
  keyword      = {transient absorption,porphyrins,enzyme catalysis,photophysics,spectroscopy,UV/Vis spectroscopy},
  language     = {eng},
  number       = {1},
  pages        = {144--150},
  publisher    = {John Wiley & Sons},
  series       = {ChemPhysChem},
  title        = {Protochlorophyllide a: A Comprehensive Photophysical Picture},
  url          = {http://dx.doi.org/10.1002/cphc.200800536},
  volume       = {10},
  year         = {2009},
}