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Protochlorophyllide a: A Comprehensive Photophysical Picture

Dietzek, Benjamin ; Tschierlei, Stefanie ; Hermann, Gudrun ; Yartsev, Arkady LU orcid ; Pascher, Torbjörn LU ; Sundström, Villy LU ; Schmitt, Michael and Popp, Juergen (2009) In ChemPhysChem 10(1). p.144-150
Abstract
The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed... (More)
The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
transient absorption, porphyrins, enzyme catalysis, photophysics, spectroscopy, UV/Vis spectroscopy
in
ChemPhysChem
volume
10
issue
1
pages
144 - 150
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000262870500020
  • scopus:58149506584
  • pmid:18855967
ISSN
1439-7641
DOI
10.1002/cphc.200800536
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
id
676fbd97-4d4a-411a-8352-447a194ffffa (old id 1311577)
date added to LUP
2016-04-01 11:33:58
date last changed
2022-03-12 21:39:10
@article{676fbd97-4d4a-411a-8352-447a194ffffa,
  abstract     = {{The photochemistry of protochlorophyllide a, a precursor in the biosynthesis of chlorophyll and substrate of the light regulated enzyme protochlorophyllide oxidoreductase, is investigated by pump-probe spectroscopy. Upon excitation into the lowest lying Q-band the light induced changes ore recorded over a wide range of probe wavelengths in the visible and near-IR region between 500 and 1000 nm. Following excitation, an initial ultrafast 450 Is process is observed related to the motion out of the Franck-Condon region on the excited state surface; thus directly unraveling previous suggestions based on time-resolved fluorescence measurements (ChemPhysChem 2006, 7, 1727-1733). Furthermore, the data reveals a previously concealed photointermediate, whose formation on a nanosecond timescale matches the overall fluorescence decay and is assigned to a triplet state. The implications of this finding with respect to the photochemistry of NADPH.-protochlorophyllide oxidoreductase (POR) ore discussed.}},
  author       = {{Dietzek, Benjamin and Tschierlei, Stefanie and Hermann, Gudrun and Yartsev, Arkady and Pascher, Torbjörn and Sundström, Villy and Schmitt, Michael and Popp, Juergen}},
  issn         = {{1439-7641}},
  keywords     = {{transient absorption; porphyrins; enzyme catalysis; photophysics; spectroscopy; UV/Vis spectroscopy}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{144--150}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{ChemPhysChem}},
  title        = {{Protochlorophyllide a: A Comprehensive Photophysical Picture}},
  url          = {{http://dx.doi.org/10.1002/cphc.200800536}},
  doi          = {{10.1002/cphc.200800536}},
  volume       = {{10}},
  year         = {{2009}},
}