Chronic intermittent L-DOPA treatment induces changes in dopamine release
Lundblad, Martin LU ; af Bjerken, Sara ; Cenci Nilsson, Angela LU
; Pomerleau, Francois
; Gerhardt, Greg A.
and Stromberg, Ingrid
(2009)
In Journal of Neurochemistry
108(4).
p.998-1008
- Abstract
- 3,4-Dihydroxyphenyl-l-alanine (l-DOPA)-induced dyskinesia often develops as a side effect of chronic l-DOPA therapy. This study was undertaken to investigate dopamine (DA) release upon l-DOPA treatment. Chronoamperometric measurements were performed in unilaterally DA-depleted rats, chronically treated with l-DOPA, resulting in dyskinetic and non-dyskinetic animals. Normal and lesioned l-DOPA naive animals were used as controls. Potassium-evoked DA releases were significantly reduced in intact sides of animals undertaken chronic l-DOPA treatment, independent on dyskinetic behavior. Acute l-DOPA further attenuated the amplitude of the DA release in the control sides. In DA-depleted striata, no difference was found in potassium-evoked DA... (More)
- 3,4-Dihydroxyphenyl-l-alanine (l-DOPA)-induced dyskinesia often develops as a side effect of chronic l-DOPA therapy. This study was undertaken to investigate dopamine (DA) release upon l-DOPA treatment. Chronoamperometric measurements were performed in unilaterally DA-depleted rats, chronically treated with l-DOPA, resulting in dyskinetic and non-dyskinetic animals. Normal and lesioned l-DOPA naive animals were used as controls. Potassium-evoked DA releases were significantly reduced in intact sides of animals undertaken chronic l-DOPA treatment, independent on dyskinetic behavior. Acute l-DOPA further attenuated the amplitude of the DA release in the control sides. In DA-depleted striata, no difference was found in potassium-evoked DA releases, and acute l-DOPA did not affect the amplitude. While immunoreactivity to serotonin uptake transporter was higher in lesioned striata of animals displaying dyskinetic behavior, no correlation could be documented between serotonin transporter-positive nerve fiber density and the amplitude of released DA. In conclusions, the amplitude of potassium-evoked DA release is attenuated in intact striatum after chronic intermittent l-DOPA treatment. No change in amplitude was found in DA-denervated sides of either dyskinetic or non-dyskinetic animals, while release kinetics were changed. This indicates the importance of studying DA release dynamics for the understanding of both beneficial and adverse effects of l-DOPA replacement therapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1312523
- author
- Lundblad, Martin
LU
; af Bjerken, Sara
; Cenci Nilsson, Angela
LU
; Pomerleau, Francois
; Gerhardt, Greg A.
and Stromberg, Ingrid
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- chronoamperometry, dopamine, serotonin transporter, 5-hydroxytryptamine or serotonin, dyskinesia, 4-dihydroxyphenyl-l-alanine induced, 3, 4-dihydroxyphenyl-l-alanine
- in
- Journal of Neurochemistry
- volume
- 108
- issue
- 4
- pages
- 998 - 1008
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000262517100014
- scopus:58549109655
- pmid:19196428
- ISSN
- 1471-4159
- DOI
- 10.1111/j.1471-4159.2008.05848.x
- language
- English
- LU publication?
- yes
- id
- 0f1e21a6-98fa-4b9e-bb49-61b0b32a36fa (old id 1312523)
- date added to LUP
- 2016-04-01 14:21:36
- date last changed
- 2025-04-04 14:36:27
@article{0f1e21a6-98fa-4b9e-bb49-61b0b32a36fa,
abstract = {{3,4-Dihydroxyphenyl-l-alanine (l-DOPA)-induced dyskinesia often develops as a side effect of chronic l-DOPA therapy. This study was undertaken to investigate dopamine (DA) release upon l-DOPA treatment. Chronoamperometric measurements were performed in unilaterally DA-depleted rats, chronically treated with l-DOPA, resulting in dyskinetic and non-dyskinetic animals. Normal and lesioned l-DOPA naive animals were used as controls. Potassium-evoked DA releases were significantly reduced in intact sides of animals undertaken chronic l-DOPA treatment, independent on dyskinetic behavior. Acute l-DOPA further attenuated the amplitude of the DA release in the control sides. In DA-depleted striata, no difference was found in potassium-evoked DA releases, and acute l-DOPA did not affect the amplitude. While immunoreactivity to serotonin uptake transporter was higher in lesioned striata of animals displaying dyskinetic behavior, no correlation could be documented between serotonin transporter-positive nerve fiber density and the amplitude of released DA. In conclusions, the amplitude of potassium-evoked DA release is attenuated in intact striatum after chronic intermittent l-DOPA treatment. No change in amplitude was found in DA-denervated sides of either dyskinetic or non-dyskinetic animals, while release kinetics were changed. This indicates the importance of studying DA release dynamics for the understanding of both beneficial and adverse effects of l-DOPA replacement therapy.}},
author = {{Lundblad, Martin and af Bjerken, Sara and Cenci Nilsson, Angela and Pomerleau, Francois and Gerhardt, Greg A. and Stromberg, Ingrid}},
issn = {{1471-4159}},
keywords = {{chronoamperometry; dopamine; serotonin transporter; 5-hydroxytryptamine or serotonin; dyskinesia; 4-dihydroxyphenyl-l-alanine induced; 3; 4-dihydroxyphenyl-l-alanine}},
language = {{eng}},
number = {{4}},
pages = {{998--1008}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Neurochemistry}},
title = {{Chronic intermittent L-DOPA treatment induces changes in dopamine release}},
url = {{http://dx.doi.org/10.1111/j.1471-4159.2008.05848.x}},
doi = {{10.1111/j.1471-4159.2008.05848.x}},
volume = {{108}},
year = {{2009}},
}