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Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays

Reisner, Walter; Larsen, Niels B.; Flyvbjerg, Henrik; Tegenfeldt, Jonas LU and Kristensen, Anders (2009) In Proceedings of the National Academy of Sciences 106(1). p.79-84
Abstract
We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into "connect-the-dots" conformations consisting of locally pinned segments joined by fluctuating linkers. These results have broad implications for nanotechnology fields that require methods for the nanoscale positioning and manipulation of DNA.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DNA conformation, self-assembly, nanofluidics, polymer confinement
in
Proceedings of the National Academy of Sciences
volume
106
issue
1
pages
79 - 84
publisher
National Acad Sciences
external identifiers
  • wos:000262263900018
  • scopus:58549094707
ISSN
1091-6490
DOI
10.1073/pnas.0811468106
language
English
LU publication?
yes
id
397c668c-1d36-40c3-8d10-bb45b366cdfe (old id 1312966)
date added to LUP
2009-03-13 08:52:10
date last changed
2017-10-29 03:38:14
@article{397c668c-1d36-40c3-8d10-bb45b366cdfe,
  abstract     = {We show that arrays of nanopit structures etched in a nanoslit can control the positioning and conformation of single DNA molecules in nanofluidic devices. By adjusting the spacing, organization and placement of the nanopits it is possible to immobilize DNA at predetermined regions of a device without additional chemical modification and achieve a high degree of control over local DNA conformation. DNA can be extended between two nanopits and in closely spaced arrays will self-assemble into "connect-the-dots" conformations consisting of locally pinned segments joined by fluctuating linkers. These results have broad implications for nanotechnology fields that require methods for the nanoscale positioning and manipulation of DNA.},
  author       = {Reisner, Walter and Larsen, Niels B. and Flyvbjerg, Henrik and Tegenfeldt, Jonas and Kristensen, Anders},
  issn         = {1091-6490},
  keyword      = {DNA conformation,self-assembly,nanofluidics,polymer confinement},
  language     = {eng},
  number       = {1},
  pages        = {79--84},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Directed self-organization of single DNA molecules in a nanoslit via embedded nanopit arrays},
  url          = {http://dx.doi.org/10.1073/pnas.0811468106},
  volume       = {106},
  year         = {2009},
}