Serological relationship between cutaneous human papillomavirus types 5, 8 and 92
(2009) In Journal of General Virology 90(1). p.136-143- Abstract
- Evidence of a possible association of cutaneous human papillomavirus (HPV) types, especially members of the genus Betapapillomavirus, and the development of non-melanoma skin cancer (NMSC) is accumulating. Vaccination with virus-like particles (VLPs) consisting of self-assembled L1, the major capsid protein, has been introduced to control anogenital HPV infection. This study examined the serological relationship between betapapillomavirus (beta 3-PV) types 5 and 8 and the new type HPV-92, which has recently been isolated from a basal cell carcinoma containing a high number of viral genomes. Following expression by recombinant baculoviruses, the L1 protein of HPV-92 self-assembled into VLPs that elicited high-titre antibodies after... (More)
- Evidence of a possible association of cutaneous human papillomavirus (HPV) types, especially members of the genus Betapapillomavirus, and the development of non-melanoma skin cancer (NMSC) is accumulating. Vaccination with virus-like particles (VLPs) consisting of self-assembled L1, the major capsid protein, has been introduced to control anogenital HPV infection. This study examined the serological relationship between betapapillomavirus (beta 3-PV) types 5 and 8 and the new type HPV-92, which has recently been isolated from a basal cell carcinoma containing a high number of viral genomes. Following expression by recombinant baculoviruses, the L1 protein of HPV-92 self-assembled into VLPs that elicited high-titre antibodies after immunization, similar to VLPs from HPV-5 and -8. Haemagglutination inhibition (HAI) assays were used as a surrogate method for the detection of virion-neutralizing antibodies, which correlates with protection from infection. Antisera raised against HPV-5 and -8 VLPs displayed HAI activity not only against the homologous type, but also against heterologous HPV types 5, 8 and 92, whereas HAI activity of antisera against HPV-92 VLP was restricted to the homologous type. The results of neutralization assays using HPV-5 pseudovirions were consistent with those from HAI assays. Cross-neutralizing immune responses by VLP vaccination against heterologous HPV types may provide broader protection against the multiplicity of HPV types detected in NMSC. If a close link to HPV infection can be conclusively established, these results may provide a basis for further evaluation of VLPs of beta-PVs as candidates for a prophylactic skin-type HPV vaccine, aimed at reducing the incidence of NMSC. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1313335
- author
- Handisurya, Alessandra ; Gambhira, Ratish ; Schellenbacher, Christina ; Shafti-Keramat, Saeed ; Forslund, Ola LU ; Favre, Michel and Kimbauer, Reinhard
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of General Virology
- volume
- 90
- issue
- 1
- pages
- 136 - 143
- publisher
- Microbiology Society
- external identifiers
-
- wos:000262263000015
- scopus:59849127953
- pmid:19088282
- ISSN
- 1465-2099
- DOI
- 10.1099/vir.0.006189-0
- language
- English
- LU publication?
- yes
- id
- d98ac248-518f-463b-bc75-c131150815d0 (old id 1313335)
- date added to LUP
- 2016-04-01 13:28:10
- date last changed
- 2025-04-04 15:15:05
@article{d98ac248-518f-463b-bc75-c131150815d0,
abstract = {{Evidence of a possible association of cutaneous human papillomavirus (HPV) types, especially members of the genus Betapapillomavirus, and the development of non-melanoma skin cancer (NMSC) is accumulating. Vaccination with virus-like particles (VLPs) consisting of self-assembled L1, the major capsid protein, has been introduced to control anogenital HPV infection. This study examined the serological relationship between betapapillomavirus (beta 3-PV) types 5 and 8 and the new type HPV-92, which has recently been isolated from a basal cell carcinoma containing a high number of viral genomes. Following expression by recombinant baculoviruses, the L1 protein of HPV-92 self-assembled into VLPs that elicited high-titre antibodies after immunization, similar to VLPs from HPV-5 and -8. Haemagglutination inhibition (HAI) assays were used as a surrogate method for the detection of virion-neutralizing antibodies, which correlates with protection from infection. Antisera raised against HPV-5 and -8 VLPs displayed HAI activity not only against the homologous type, but also against heterologous HPV types 5, 8 and 92, whereas HAI activity of antisera against HPV-92 VLP was restricted to the homologous type. The results of neutralization assays using HPV-5 pseudovirions were consistent with those from HAI assays. Cross-neutralizing immune responses by VLP vaccination against heterologous HPV types may provide broader protection against the multiplicity of HPV types detected in NMSC. If a close link to HPV infection can be conclusively established, these results may provide a basis for further evaluation of VLPs of beta-PVs as candidates for a prophylactic skin-type HPV vaccine, aimed at reducing the incidence of NMSC.}},
author = {{Handisurya, Alessandra and Gambhira, Ratish and Schellenbacher, Christina and Shafti-Keramat, Saeed and Forslund, Ola and Favre, Michel and Kimbauer, Reinhard}},
issn = {{1465-2099}},
language = {{eng}},
number = {{1}},
pages = {{136--143}},
publisher = {{Microbiology Society}},
series = {{Journal of General Virology}},
title = {{Serological relationship between cutaneous human papillomavirus types 5, 8 and 92}},
url = {{http://dx.doi.org/10.1099/vir.0.006189-0}},
doi = {{10.1099/vir.0.006189-0}},
volume = {{90}},
year = {{2009}},
}