Bilirubin recognition via molecularly imprinted supermacroporous cryogels
(2009) In Colloids and Surfaces B: Biointerfaces 68(1). p.33-38- Abstract
- Recent years molecular imprinting has received considerable attention as an excellent and simple approach to recognize small molecules and bioactive substances. The aim of this study is to prepare the bilirubin-imprinted supermacroporous cryogels which can be used for the adsorption of bilirubin from human plasma. N-methacryloyl(L)-tyrosinemethylester (MAT) was chosen as the preorganization monomer. In the first step, bilirubin was complexed with MAT and the bilirubin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) [BR-MIP] cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After that, the template... (More)
- Recent years molecular imprinting has received considerable attention as an excellent and simple approach to recognize small molecules and bioactive substances. The aim of this study is to prepare the bilirubin-imprinted supermacroporous cryogels which can be used for the adsorption of bilirubin from human plasma. N-methacryloyl(L)-tyrosinemethylester (MAT) was chosen as the preorganization monomer. In the first step, bilirubin was complexed with MAT and the bilirubin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) [BR-MIP] cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After that, the template molecules (i.e., bilirubin) were removed from the polymeric structure using sodium carbonate and sodium hydroxide. The maximum bilirubin adsorption amount was 3.6 mg/g polymer. The relative selectivity coefficients of the BR-MIP cryogel for bilirubin/cholesterol and bilirubin/testosterone mixtures were 7.3 and 3.2 times greater than non-imprinted poly(HEMA-MAT) [NIP] cryogel, respectively. The BR-MIP cryogel could be used many times without decreasing bilirubin adsorption amount significantly. Therefore, as a reusable carrier possessing high selectivity, BR-MIP cryogel has a potential candidate as a clinical hemoperfusion material. (C) 2008 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1313687
- author
- Baydemir, Goezde ; Bereli, Nilay ; Andac, Muege ; Say, Ridvan ; Galaev, Igor LU and Denizli, Adil
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bilirubin, Molecular recognition, Cryogels, Molecular imprinting, Affinity binding
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 68
- issue
- 1
- pages
- 33 - 38
- publisher
- Elsevier
- external identifiers
-
- wos:000261980700006
- scopus:56549122038
- pmid:18976889
- ISSN
- 1873-4367
- DOI
- 10.1016/j.colsurfb.2008.09.008
- language
- English
- LU publication?
- yes
- id
- cf9a9a92-df98-4427-985e-a7608046a9e5 (old id 1313687)
- date added to LUP
- 2016-04-01 13:00:55
- date last changed
- 2022-02-19 02:17:14
@article{cf9a9a92-df98-4427-985e-a7608046a9e5,
abstract = {{Recent years molecular imprinting has received considerable attention as an excellent and simple approach to recognize small molecules and bioactive substances. The aim of this study is to prepare the bilirubin-imprinted supermacroporous cryogels which can be used for the adsorption of bilirubin from human plasma. N-methacryloyl(L)-tyrosinemethylester (MAT) was chosen as the preorganization monomer. In the first step, bilirubin was complexed with MAT and the bilirubin-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) [BR-MIP] cryogel was produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) pair in an ice bath. After that, the template molecules (i.e., bilirubin) were removed from the polymeric structure using sodium carbonate and sodium hydroxide. The maximum bilirubin adsorption amount was 3.6 mg/g polymer. The relative selectivity coefficients of the BR-MIP cryogel for bilirubin/cholesterol and bilirubin/testosterone mixtures were 7.3 and 3.2 times greater than non-imprinted poly(HEMA-MAT) [NIP] cryogel, respectively. The BR-MIP cryogel could be used many times without decreasing bilirubin adsorption amount significantly. Therefore, as a reusable carrier possessing high selectivity, BR-MIP cryogel has a potential candidate as a clinical hemoperfusion material. (C) 2008 Elsevier B.V. All rights reserved.}},
author = {{Baydemir, Goezde and Bereli, Nilay and Andac, Muege and Say, Ridvan and Galaev, Igor and Denizli, Adil}},
issn = {{1873-4367}},
keywords = {{Bilirubin; Molecular recognition; Cryogels; Molecular imprinting; Affinity binding}},
language = {{eng}},
number = {{1}},
pages = {{33--38}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Bilirubin recognition via molecularly imprinted supermacroporous cryogels}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2008.09.008}},
doi = {{10.1016/j.colsurfb.2008.09.008}},
volume = {{68}},
year = {{2009}},
}