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Osmotic pressures of lysozyme solutions from gas-like to crystal states

Pasquier, Coralie LU ; Beaufils, Sylvie; Bouchoux, Antoine; Rigault, Sophie; Cabane, Bernard LU ; Lund, Mikael LU ; Lechevalier, Valérie; Le Floch-Fouéré, Cécile; Pasco, Maryvonne and Pabœuf, Gilles, et al. (2016) In Physical Chemistry Chemical Physics 18(41). p.28458-28465
Abstract

We obtained osmotic pressure data of lysozyme solutions, describing their physical states over a wide concentration range, using osmotic stress for pressures between 0.05 bar and about 40 bar and volume fractions between 0.01 and 0.61. The osmotic pressure vs. volume fraction data consist of a dilute, gas-phase regime, a transition regime with a high-compressibility plateau, and a concentrated regime where the system is nearly incompressible. The first two regimes are shifted towards a higher protein volume fraction upon decreasing the strength or the range of electrostatic interactions. We describe this shift and the overall shape of the experimental data in these two regimes through a model accounting for a steric repulsion, a... (More)

We obtained osmotic pressure data of lysozyme solutions, describing their physical states over a wide concentration range, using osmotic stress for pressures between 0.05 bar and about 40 bar and volume fractions between 0.01 and 0.61. The osmotic pressure vs. volume fraction data consist of a dilute, gas-phase regime, a transition regime with a high-compressibility plateau, and a concentrated regime where the system is nearly incompressible. The first two regimes are shifted towards a higher protein volume fraction upon decreasing the strength or the range of electrostatic interactions. We describe this shift and the overall shape of the experimental data in these two regimes through a model accounting for a steric repulsion, a short-range van der Waals attraction and a screened electrostatic repulsion. The transition is caused by crystallization, as shown by small-angle X-ray scattering. We verified that our data points correspond to thermodynamic equilibria, and thus that they consist of the reference experimental counterpart of a thermodynamic equation of state.

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published
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Physical Chemistry Chemical Physics
volume
18
issue
41
pages
8 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84992361717
  • wos:000386668200009
ISSN
1463-9076
DOI
10.1039/c6cp03867k
language
English
LU publication?
yes
id
1315ce00-571b-42ed-af16-c3f13843e661
date added to LUP
2017-02-21 13:53:19
date last changed
2017-09-18 11:34:05
@article{1315ce00-571b-42ed-af16-c3f13843e661,
  abstract     = {<p>We obtained osmotic pressure data of lysozyme solutions, describing their physical states over a wide concentration range, using osmotic stress for pressures between 0.05 bar and about 40 bar and volume fractions between 0.01 and 0.61. The osmotic pressure vs. volume fraction data consist of a dilute, gas-phase regime, a transition regime with a high-compressibility plateau, and a concentrated regime where the system is nearly incompressible. The first two regimes are shifted towards a higher protein volume fraction upon decreasing the strength or the range of electrostatic interactions. We describe this shift and the overall shape of the experimental data in these two regimes through a model accounting for a steric repulsion, a short-range van der Waals attraction and a screened electrostatic repulsion. The transition is caused by crystallization, as shown by small-angle X-ray scattering. We verified that our data points correspond to thermodynamic equilibria, and thus that they consist of the reference experimental counterpart of a thermodynamic equation of state.</p>},
  author       = {Pasquier, Coralie and Beaufils, Sylvie and Bouchoux, Antoine and Rigault, Sophie and Cabane, Bernard and Lund, Mikael and Lechevalier, Valérie and Le Floch-Fouéré, Cécile and Pasco, Maryvonne and Pabœuf, Gilles and Pérez, Javier and Pezennec, Stéphane},
  issn         = {1463-9076},
  language     = {eng},
  number       = {41},
  pages        = {28458--28465},
  publisher    = {Royal Society of Chemistry},
  series       = {Physical Chemistry Chemical Physics},
  title        = {Osmotic pressures of lysozyme solutions from gas-like to crystal states},
  url          = {http://dx.doi.org/10.1039/c6cp03867k},
  volume       = {18},
  year         = {2016},
}