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2,3-butanedione monoxime increases speed of relaxation in single muscle fibres of frog

Sun, Y-B.; Lou, F. and Edman, Paul LU (2001) In Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00 172(1). p.53-61
Abstract
The effects of 2,3-butanedione monoxime (BDM) on intracellular Ca2+ transient and cross-bridge function were studied in frog single fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 m; temperature, 24 C). The fluorescent dye fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+i) during isometric contractions. BDM (15 m M) reduced the amplitude of the Ca2+ transient during twitches, but this effect was too small to explain the marked inhibition of BDM on twitch force. [Ca2+i reached at the end of 1-s tetanic stimulation was not significantly affected by BDM (1.0 and 1.8 m M) while the maximum tetanic tension was substantially reduced. The rate of relaxation during isometric tetanus... (More)
The effects of 2,3-butanedione monoxime (BDM) on intracellular Ca2+ transient and cross-bridge function were studied in frog single fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 m; temperature, 24 C). The fluorescent dye fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+i) during isometric contractions. BDM (15 m M) reduced the amplitude of the Ca2+ transient during twitches, but this effect was too small to explain the marked inhibition of BDM on twitch force. [Ca2+i reached at the end of 1-s tetanic stimulation was not significantly affected by BDM (1.0 and 1.8 m M) while the maximum tetanic tension was substantially reduced. The rate of relaxation during isometric tetanus was increased by BDM whereas the rate of decay of the Ca2+ transient was reduced in the presence of BDM. The results strongly suggest that BDM, under the experimental conditions used, mainly affects the contractile machinery resulting in altered performance of the cross-bridges. These effects of BDM were evaluated in terms of the cross-bridge model of Huxley (1957) which was fitted to the experimental forcevelocity data in the presence and absence of BDM. (Less)
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Contribution to journal
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published
subject
in
Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00
volume
172
issue
1
pages
53 - 61
publisher
Wiley-Blackwell
external identifiers
  • wos:000169891700006
  • scopus:0034818970
ISSN
0001-6772
DOI
10.1046/j.1365-201X.2001.00818.x
language
English
LU publication?
yes
id
91827c37-459c-4ebd-8155-b705e6e4ab40 (old id 131804)
date added to LUP
2007-07-19 14:57:10
date last changed
2018-05-29 12:14:00
@article{91827c37-459c-4ebd-8155-b705e6e4ab40,
  abstract     = {The effects of 2,3-butanedione monoxime (BDM) on intracellular Ca2+ transient and cross-bridge function were studied in frog single fibres from the anterior tibialis muscle of Rana temporaria (sarcomere length, 2.2 m; temperature, 24 C). The fluorescent dye fluo-3 was used to monitor the intracellular free calcium concentration ([Ca2+i) during isometric contractions. BDM (15 m M) reduced the amplitude of the Ca2+ transient during twitches, but this effect was too small to explain the marked inhibition of BDM on twitch force. [Ca2+i reached at the end of 1-s tetanic stimulation was not significantly affected by BDM (1.0 and 1.8 m M) while the maximum tetanic tension was substantially reduced. The rate of relaxation during isometric tetanus was increased by BDM whereas the rate of decay of the Ca2+ transient was reduced in the presence of BDM. The results strongly suggest that BDM, under the experimental conditions used, mainly affects the contractile machinery resulting in altered performance of the cross-bridges. These effects of BDM were evaluated in terms of the cross-bridge model of Huxley (1957) which was fitted to the experimental forcevelocity data in the presence and absence of BDM.},
  author       = {Sun, Y-B. and Lou, F. and Edman, Paul},
  issn         = {0001-6772},
  language     = {eng},
  number       = {1},
  pages        = {53--61},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica1888-12-31+01:002006-01-01+01:00},
  title        = {2,3-butanedione monoxime increases speed of relaxation in single muscle fibres of frog},
  url          = {http://dx.doi.org/10.1046/j.1365-201X.2001.00818.x},
  volume       = {172},
  year         = {2001},
}