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Phenotypic variation of Helicobacter pylori isolates from geographically distinct regions detected by lectin typing

Hynes, Sean LU ; Broutet, Nathalie; Eurohepygast Study Group, The; Wadström, Torkel LU ; Mikelsaar, Marika; O’Toole, Paul W.; Telford, John; Engstrand, Lars; Kamiya, Shigeru and Mentis, Andreas F., et al. (2002) In Journal of Clinical Microbiology 40(1). p.227-232
Abstract
A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cristigali, and Triticum vulgaris were used to type the strains. The lectins were chosen for their specificities for sugars commonly encountered in the lipopolysaccharide of H. pylori. The isolates were received from their parent institutions as pellets of biomass and were typed at one of three centers (in Ireland, Sweden, and Estonia). All 16 possible lectin reaction patterns were observed in the study, with the isolates with the... (More)
A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cristigali, and Triticum vulgaris were used to type the strains. The lectins were chosen for their specificities for sugars commonly encountered in the lipopolysaccharide of H. pylori. The isolates were received from their parent institutions as pellets of biomass and were typed at one of three centers (in Ireland, Sweden, and Estonia). All 16 possible lectin reaction patterns were observed in the study, with the isolates with the predominant pattern exhibiting reactions with all the lectins in the panel. For European patients suffering from gastritis, an association was noted between lectin reaction pattern MH4 and atrophic chronic gastritis; isolates with lectin reaction pattern MH4 were isolated from patients with atrophic chronic gastritis, whereas isolates with this pattern were not isolated from patients with chronic gastritis (P = 0.0006). In addition, statistically significant relationships were noted between the lectin reaction pattern and the associated pathology of isolates from the Swedish population. Isolates with patterns MH13 and MH16, which had low lectin reactivities, correlated with nonulcer disease (P = 0.0025 and P = 0.0002, respectively), and all four isolates from adenocarcinoma patients were characterized as possessing reaction pattern MH16. In contrast, isolates with lectin reaction patterns MH1 and MH10, which had high lectin reactivities, were associated with ulcer disease (P = 0.046 and P = 0.0022, respectively). (Less)
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Journal of Clinical Microbiology
volume
40
issue
1
pages
227 - 232
publisher
American Society for Microbiology
external identifiers
  • wos:000173121800038
  • pmid:11773120
  • scopus:0036136908
ISSN
1098-660X
DOI
10.1128/JCM.40.1.227-232.2002
language
English
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yes
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4c1138ca-634c-4bb7-9c27-30bf2b6f9245 (old id 132344)
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2007-07-10 07:06:28
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2017-01-01 06:44:57
@article{4c1138ca-634c-4bb7-9c27-30bf2b6f9245,
  abstract     = {A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cristigali, and Triticum vulgaris were used to type the strains. The lectins were chosen for their specificities for sugars commonly encountered in the lipopolysaccharide of H. pylori. The isolates were received from their parent institutions as pellets of biomass and were typed at one of three centers (in Ireland, Sweden, and Estonia). All 16 possible lectin reaction patterns were observed in the study, with the isolates with the predominant pattern exhibiting reactions with all the lectins in the panel. For European patients suffering from gastritis, an association was noted between lectin reaction pattern MH4 and atrophic chronic gastritis; isolates with lectin reaction pattern MH4 were isolated from patients with atrophic chronic gastritis, whereas isolates with this pattern were not isolated from patients with chronic gastritis (P = 0.0006). In addition, statistically significant relationships were noted between the lectin reaction pattern and the associated pathology of isolates from the Swedish population. Isolates with patterns MH13 and MH16, which had low lectin reactivities, correlated with nonulcer disease (P = 0.0025 and P = 0.0002, respectively), and all four isolates from adenocarcinoma patients were characterized as possessing reaction pattern MH16. In contrast, isolates with lectin reaction patterns MH1 and MH10, which had high lectin reactivities, were associated with ulcer disease (P = 0.046 and P = 0.0022, respectively).},
  author       = {Hynes, Sean and Broutet, Nathalie and Eurohepygast Study Group, The and Wadström, Torkel and Mikelsaar, Marika and O’Toole, Paul W. and Telford, John and Engstrand, Lars and Kamiya, Shigeru and Mentis, Andreas F. and Moran, Anthony P.},
  issn         = {1098-660X},
  language     = {eng},
  number       = {1},
  pages        = {227--232},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Phenotypic variation of Helicobacter pylori isolates from geographically distinct regions detected by lectin typing},
  url          = {http://dx.doi.org/10.1128/JCM.40.1.227-232.2002},
  volume       = {40},
  year         = {2002},
}