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Modes of adherence of Helicobacter pylori to gastric surface epithelium in gastroduodenal disease: A possible sequence of events leading to internalisation

Papadogiannakis, Nikos; Willén, Roger; Carlén, Birgitta LU ; Sjöstedt, Svante; Wadström, Torkel LU and Gad, Adel (2000) In Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS) 108(6). p.439-447
Abstract
We have investigated various modes of adherence of Helicobacter pylori to the human gastric epithelium, using transmission electron microscopy, in biopsies from nine patients with peptic ulcer disease and from four patients with chronic active gastritis. H. pylori was demonstrated in abundance in all cases within the surface mucous layer. In all ulcer- and in one out of four gastritis patients H. pylori was shown in close proximity to the gastric epithelium, with concurrent alterations in the configuration of microvilli and the apical cytoplasmic region of gastric cells. Previously described modes of H. pylori adherence were confirmed, such as loose attachment with fibrillar-like strands, firm attachment with pedestal formation, invasion... (More)
We have investigated various modes of adherence of Helicobacter pylori to the human gastric epithelium, using transmission electron microscopy, in biopsies from nine patients with peptic ulcer disease and from four patients with chronic active gastritis. H. pylori was demonstrated in abundance in all cases within the surface mucous layer. In all ulcer- and in one out of four gastritis patients H. pylori was shown in close proximity to the gastric epithelium, with concurrent alterations in the configuration of microvilli and the apical cytoplasmic region of gastric cells. Previously described modes of H. pylori adherence were confirmed, such as loose attachment with fibrillar-like strands, firm attachment with pedestal formation, invasion in the intercellular spaces, and invagination with cup formation. Moreover, in many cases a fusion between the bacterial outer layer and gastric cell membranes was evident. In four cases (31; three with active and one with past ulcer disease) viable H. pylori was found in the cytoplasm of gastric mucous cells. Our results support the hypothesis that the different modes of adherence of H. pylori represent a stepwise, possibly sequential, process which in a significant number of cases leads to internalisation of the organism. The invariable occurrence of adhesion and more frequent internalisation of H. pylori in ulcer patients may suggest a link with the pathogenesis of peptic ulcer disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
volume
108
issue
6
pages
439 - 447
publisher
John Wiley & Sons
external identifiers
  • wos:000089075900007
ISSN
1600-0463
DOI
10.1034/j.1600-0463.2000.d01-80.x
language
English
LU publication?
yes
id
441d658e-f3bd-43b7-894f-5bea43e9a81b (old id 132362)
date added to LUP
2007-07-10 12:44:56
date last changed
2016-04-15 18:37:45
@article{441d658e-f3bd-43b7-894f-5bea43e9a81b,
  abstract     = {We have investigated various modes of adherence of Helicobacter pylori to the human gastric epithelium, using transmission electron microscopy, in biopsies from nine patients with peptic ulcer disease and from four patients with chronic active gastritis. H. pylori was demonstrated in abundance in all cases within the surface mucous layer. In all ulcer- and in one out of four gastritis patients H. pylori was shown in close proximity to the gastric epithelium, with concurrent alterations in the configuration of microvilli and the apical cytoplasmic region of gastric cells. Previously described modes of H. pylori adherence were confirmed, such as loose attachment with fibrillar-like strands, firm attachment with pedestal formation, invasion in the intercellular spaces, and invagination with cup formation. Moreover, in many cases a fusion between the bacterial outer layer and gastric cell membranes was evident. In four cases (31; three with active and one with past ulcer disease) viable H. pylori was found in the cytoplasm of gastric mucous cells. Our results support the hypothesis that the different modes of adherence of H. pylori represent a stepwise, possibly sequential, process which in a significant number of cases leads to internalisation of the organism. The invariable occurrence of adhesion and more frequent internalisation of H. pylori in ulcer patients may suggest a link with the pathogenesis of peptic ulcer disease.},
  author       = {Papadogiannakis, Nikos and Willén, Roger and Carlén, Birgitta and Sjöstedt, Svante and Wadström, Torkel and Gad, Adel},
  issn         = {1600-0463},
  language     = {eng},
  number       = {6},
  pages        = {439--447},
  publisher    = {John Wiley & Sons},
  series       = {Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)},
  title        = {Modes of adherence of Helicobacter pylori to gastric surface epithelium in gastroduodenal disease: A possible sequence of events leading to internalisation},
  url          = {http://dx.doi.org/10.1034/j.1600-0463.2000.d01-80.x},
  volume       = {108},
  year         = {2000},
}