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Detection of Helicobacter ganmani-Like 16S rDNA in Pediatric Liver Tissue

Tolia, Vasundhara; Nilsson, Hans-Olof LU ; Boyer, Kimberly; Wuerth, Anne; Abu Al-Soud, Waleed LU ; Rabah, Raja and Wadström, Torkel LU (2004) In Helicobacter 9(5). p.460-468
Abstract
Background. To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis.Materials and methods. Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects with no evidence of preexisting liver disease were examined for the presence of Helicobacter species by a genus-specific seminested polymerase chain reaction (PCR) assay. PCRproducts of positive samples were further characterized by denaturing gradient gel electrophoresis (DGGE) and DNA-sequence analysis. Based on those results, a seminested PCR... (More)
Background. To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis.Materials and methods. Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects with no evidence of preexisting liver disease were examined for the presence of Helicobacter species by a genus-specific seminested polymerase chain reaction (PCR) assay. PCRproducts of positive samples were further characterized by denaturing gradient gel electrophoresis (DGGE) and DNA-sequence analysis. Based on those results, a seminested PCR assay for H. ganmani was developed and applied to the samples.Results. On analysis, 40/61 patient samples were positive in the genus-specific Helicobacter PCR and 4/10 from the control group. The nucleotide sequences of 16S rDNA fragments were 99100 similar to mainly Helicobacter sp. liver and H. ganmani. PCR-products similar to H. canis and H. bilis were also found. The 16S rDNAs of control specimens showed similarity to Helicobacter sp. liver. In the H. ganmani-specific PCR analysis 19 patients, but none of the controls, were positive.Conclusions. Amplified Helicobacter 16S rDNAs were related to Helicobacter sp. liver or H. ganmani in liver biopsy specimens of pediatric patients. The possible significance of Helicobacter species in pediatric liver diseases needs to be evaluated further in prospective studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Helicobacter
volume
9
issue
5
pages
460 - 468
publisher
Wiley-Blackwell
external identifiers
  • pmid:15361086
  • wos:000223781200012
  • scopus:4644371290
ISSN
1083-4389
DOI
10.1111/j.1083-4389.2004.00266.x
language
English
LU publication?
yes
id
ade0d92a-21bc-4a2c-b301-ac2320964a92 (old id 132425)
date added to LUP
2007-07-10 14:29:10
date last changed
2017-01-01 04:43:27
@article{ade0d92a-21bc-4a2c-b301-ac2320964a92,
  abstract     = {Background. To determine the presence of Helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis.Materials and methods. Paraffin sections of 61 liver biopsies of pediatric patients with miscellaneous diseases and autopsy liver tissue from 10 control subjects with no evidence of preexisting liver disease were examined for the presence of Helicobacter species by a genus-specific seminested polymerase chain reaction (PCR) assay. PCRproducts of positive samples were further characterized by denaturing gradient gel electrophoresis (DGGE) and DNA-sequence analysis. Based on those results, a seminested PCR assay for H. ganmani was developed and applied to the samples.Results. On analysis, 40/61 patient samples were positive in the genus-specific Helicobacter PCR and 4/10 from the control group. The nucleotide sequences of 16S rDNA fragments were 99100 similar to mainly Helicobacter sp. liver and H. ganmani. PCR-products similar to H. canis and H. bilis were also found. The 16S rDNAs of control specimens showed similarity to Helicobacter sp. liver. In the H. ganmani-specific PCR analysis 19 patients, but none of the controls, were positive.Conclusions. Amplified Helicobacter 16S rDNAs were related to Helicobacter sp. liver or H. ganmani in liver biopsy specimens of pediatric patients. The possible significance of Helicobacter species in pediatric liver diseases needs to be evaluated further in prospective studies.},
  author       = {Tolia, Vasundhara and Nilsson, Hans-Olof and Boyer, Kimberly and Wuerth, Anne and Abu Al-Soud, Waleed and Rabah, Raja and Wadström, Torkel},
  issn         = {1083-4389},
  language     = {eng},
  number       = {5},
  pages        = {460--468},
  publisher    = {Wiley-Blackwell},
  series       = {Helicobacter},
  title        = {Detection of Helicobacter ganmani-Like 16S rDNA in Pediatric Liver Tissue},
  url          = {http://dx.doi.org/10.1111/j.1083-4389.2004.00266.x},
  volume       = {9},
  year         = {2004},
}