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A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction

Liden, A ; Karlstrom, A ; Lannergard, J ; Kalamajski, Sebastian LU ; Guss, B ; Rubin, K and Ryden, C (2006) In Biochemical and Biophysical Research Communications 340(2). p.604-610
Abstract
The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemieus was investigated as to effects on murine cell interactions with extracellular matrix proteins, FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alpha V beta 3. C2C12 cells lack collagen-binding beta 1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel... (More)
The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemieus was investigated as to effects on murine cell interactions with extracellular matrix proteins, FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alpha V beta 3. C2C12 cells lack collagen-binding beta 1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alpha V beta 3-inhibitor suggested that FNZN promoted contraction by it process requiring alpha V beta 3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alpha V beta 3 integrin, Since alpha V beta 3-mediated contractile events function to counteract edema formation during inflammation. it is possible that FNZN and its secreted homologue FINE modulate edema responses in infected tissues. (c) 2005 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
infection, adhesion, edema, collagen structure, collagen-binding, integrins
in
Biochemical and Biophysical Research Communications
volume
340
issue
2
pages
604 - 610
publisher
Elsevier
external identifiers
  • pmid:16376297
  • wos:000234731800036
  • scopus:29644445323
ISSN
1090-2104
DOI
10.1016/j.bbrc.2005.12.043
language
English
LU publication?
yes
id
132849b1-5f26-4585-b03f-5e4b3b3fe8cb (old id 419965)
date added to LUP
2016-04-01 15:59:38
date last changed
2020-01-12 18:56:07
@article{132849b1-5f26-4585-b03f-5e4b3b3fe8cb,
  abstract     = {The N-terminal fragment (FNZN) of the fibronectin-binding protein FNZ from Streptococcus equi subspecies zooepidemieus was investigated as to effects on murine cell interactions with extracellular matrix proteins, FNZN bound to immobilized fibronectin (FN) and native, but not denatured, collagen type I. FNZN had no effect on primary adhesion of cells from the murine myoblastic C2C12 cell line to immobilized fibronectin. C2C12 cells adhered to immobilized FNZN, a process that was not inhibited by anti-human FN IgG or by an inhibitor of integrin alpha V beta 3. C2C12 cells lack collagen-binding beta 1 integrins and neither adhere to native collagen nor mediate contraction of three-dimensional collagen gels. FNZN stimulated collagen gel contraction by C2C12 cells but not adhesion of C2C12 cells to collagen. Experiments with an alpha V beta 3-inhibitor suggested that FNZN promoted contraction by it process requiring alpha V beta 3. Our data suggest that FNZN by binding to cells, collagen, and FN modulate complex adhesive processes mediated by the alpha V beta 3 integrin, Since alpha V beta 3-mediated contractile events function to counteract edema formation during inflammation. it is possible that FNZN and its secreted homologue FINE modulate edema responses in infected tissues. (c) 2005 Elsevier Inc. All rights reserved.},
  author       = {Liden, A and Karlstrom, A and Lannergard, J and Kalamajski, Sebastian and Guss, B and Rubin, K and Ryden, C},
  issn         = {1090-2104},
  language     = {eng},
  number       = {2},
  pages        = {604--610},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {A fibronectin-binding protein from Streptococcus equi binds collagen and modulates cell-mediated collagen gel contraction},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2005.12.043},
  doi          = {10.1016/j.bbrc.2005.12.043},
  volume       = {340},
  year         = {2006},
}