Use of phospholipid fatty acids to detect previous self-heating events in stored peat
(2003) In Applied and Environmental Microbiology 69(6). p.3532-3539- Abstract
- The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat. Two PLFAs, denoted T1 and T2, were detected only in the self-heated peat. Incubation of peat samples at 25 to 55°C for 4 days indicated that T1 and T2 were produced from microorganisms with different optimum temperatures. This was confirmed by isolation of bacteria at 55°C, which produced T2 but not T1. These bacteria produced another PLFA (denoted T3) which coeluted with 18:1{omega}7. T2 and T3 were identified as... (More)
- The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat. Two PLFAs, denoted T1 and T2, were detected only in the self-heated peat. Incubation of peat samples at 25 to 55°C for 4 days indicated that T1 and T2 were produced from microorganisms with different optimum temperatures. This was confirmed by isolation of bacteria at 55°C, which produced T2 but not T1. These bacteria produced another PLFA (denoted T3) which coeluted with 18:1{omega}7. T2 and T3 were identified as {omega}-cyclohexyltridecanoic acid and {omega}-cyclohexylundecanoic acid, respectively, indicating that the bacteria belonged to the genus Alicyclobacillus. T1 was tentatively identified as {omega}-cycloheptylundecanoic acid. T2 was detected 8 h after the peat incubation temperature was increased to 55°C, and maximum levels were found within 5 days of incubation. The PLFA 18:1{omega}7-T3 increased in proportion to T2. T1 was detected after 96 h at 55°C, and its level increased throughout the incubation period, so that it eventually became one of the dominant PLFAs after 80 days. In peat samples incubated at 55°C and then at 25°C, T1 and T2 disappeared slowly. After 3 months, detectable levels were still found. Incubation at 25°C after heating for 3 days at 55°C decreased the amounts of T2 and 18:1{omega}7-T3 faster than did incubation at 5°C. Thus, not only the duration and temperature during the heating event but also the storage temperature following heating are important for the detection of PLFAs indicating previous self-heating. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132884
- author
- Ranneklev, Sissel Brit and Bååth, Erland LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 69
- issue
- 6
- pages
- 3532 - 3539
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000187156200064
- pmid:12788760
- scopus:0037640906
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.69.6.3532-3539.2003
- language
- English
- LU publication?
- yes
- id
- 8b61df42-1458-4b4d-95fa-c71f6a62b2e6 (old id 132884)
- date added to LUP
- 2016-04-01 12:10:57
- date last changed
- 2022-01-26 23:58:38
@article{8b61df42-1458-4b4d-95fa-c71f6a62b2e6,
abstract = {{The use of the phospholipid fatty acid (PLFA) composition of microorganisms to detect previous self-heating events was studied in naturally self-heated peat and in peat incubated under temperature-controlled conditions. An increased content of total PLFAs was found in self-heated peat compared to that in unheated peat. Two PLFAs, denoted T1 and T2, were detected only in the self-heated peat. Incubation of peat samples at 25 to 55°C for 4 days indicated that T1 and T2 were produced from microorganisms with different optimum temperatures. This was confirmed by isolation of bacteria at 55°C, which produced T2 but not T1. These bacteria produced another PLFA (denoted T3) which coeluted with 18:1{omega}7. T2 and T3 were identified as {omega}-cyclohexyltridecanoic acid and {omega}-cyclohexylundecanoic acid, respectively, indicating that the bacteria belonged to the genus Alicyclobacillus. T1 was tentatively identified as {omega}-cycloheptylundecanoic acid. T2 was detected 8 h after the peat incubation temperature was increased to 55°C, and maximum levels were found within 5 days of incubation. The PLFA 18:1{omega}7-T3 increased in proportion to T2. T1 was detected after 96 h at 55°C, and its level increased throughout the incubation period, so that it eventually became one of the dominant PLFAs after 80 days. In peat samples incubated at 55°C and then at 25°C, T1 and T2 disappeared slowly. After 3 months, detectable levels were still found. Incubation at 25°C after heating for 3 days at 55°C decreased the amounts of T2 and 18:1{omega}7-T3 faster than did incubation at 5°C. Thus, not only the duration and temperature during the heating event but also the storage temperature following heating are important for the detection of PLFAs indicating previous self-heating.}},
author = {{Ranneklev, Sissel Brit and Bååth, Erland}},
issn = {{0099-2240}},
language = {{eng}},
number = {{6}},
pages = {{3532--3539}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Use of phospholipid fatty acids to detect previous self-heating events in stored peat}},
url = {{https://lup.lub.lu.se/search/files/2816638/624334.pdf}},
doi = {{10.1128/AEM.69.6.3532-3539.2003}},
volume = {{69}},
year = {{2003}},
}