Laminin {alpha}1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly.
(2005) In Proceedings of the National Academy of Sciences 102(5). p.1502-1506- Abstract
- basement membrane (BM) assembly, which is required for pregastrulation development. Individual domains may have other functions, not necessarily structural. The cell binding C terminus of Lm {alpha}1 chain contains five Lm globular (LG) domains. In vitro, {alpha}1LG1–3 domains bind integrins, and {alpha}1LG4 binds dystroglycan, heparin, and sulfatides. A prevailing hypothesis is that {alpha}1LG4 is crucial as a structural domain for BM assembly, whereas integrin-binding sites conduct signaling. The in vivo role of {alpha}1LG4–5 (also called E3) has not been studied. Mice lacking {alpha}1LG4–5 were therefore made. Null embryos implanted, but presumptive epiblast cells failed to polarize and did not survive past day 6.5. BM components... (More)
- basement membrane (BM) assembly, which is required for pregastrulation development. Individual domains may have other functions, not necessarily structural. The cell binding C terminus of Lm {alpha}1 chain contains five Lm globular (LG) domains. In vitro, {alpha}1LG1–3 domains bind integrins, and {alpha}1LG4 binds dystroglycan, heparin, and sulfatides. A prevailing hypothesis is that {alpha}1LG4 is crucial as a structural domain for BM assembly, whereas integrin-binding sites conduct signaling. The in vivo role of {alpha}1LG4–5 (also called E3) has not been studied. Mice lacking {alpha}1LG4–5 were therefore made. Null embryos implanted, but presumptive epiblast cells failed to polarize and did not survive past day 6.5. BM components including truncated Lm {alpha}1 were detected in Reichert's membrane. Surprisingly, embryonic BM assembly between visceral endoderm and stem cells was normal in null embryos and in embryoid bodies of {alpha}1LG4–5-null embryonic stem cells. Yet, stem cells could not develop into polarized epiblast cells. Thus, {alpha}1LG4–5 provides vital signals for the conversion of stem cells to polarized epithelium. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/132950
- author
- Schéele, Susanne LU ; Falk, Mats ; Franzén, Ahnders LU ; Ellin, Fredrik ; Ferletta, Maria ; Lonai, Peter ; Andersson, Björn ; Timpl, Rupert ; Forsberg, Erik and Ekblom, Peter LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- epiblast, epithelial polarity, stem cells, mouse development
- in
- Proceedings of the National Academy of Sciences
- volume
- 102
- issue
- 5
- pages
- 1502 - 1506
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000226877300046
- scopus:13444265889
- pmid:15668394
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.0405095102
- language
- English
- LU publication?
- yes
- id
- a01ef637-b48b-439e-bcff-18019ba716e4 (old id 132950)
- date added to LUP
- 2016-04-01 12:35:53
- date last changed
- 2022-01-27 07:17:19
@article{a01ef637-b48b-439e-bcff-18019ba716e4, abstract = {{basement membrane (BM) assembly, which is required for pregastrulation development. Individual domains may have other functions, not necessarily structural. The cell binding C terminus of Lm {alpha}1 chain contains five Lm globular (LG) domains. In vitro, {alpha}1LG1–3 domains bind integrins, and {alpha}1LG4 binds dystroglycan, heparin, and sulfatides. A prevailing hypothesis is that {alpha}1LG4 is crucial as a structural domain for BM assembly, whereas integrin-binding sites conduct signaling. The in vivo role of {alpha}1LG4–5 (also called E3) has not been studied. Mice lacking {alpha}1LG4–5 were therefore made. Null embryos implanted, but presumptive epiblast cells failed to polarize and did not survive past day 6.5. BM components including truncated Lm {alpha}1 were detected in Reichert's membrane. Surprisingly, embryonic BM assembly between visceral endoderm and stem cells was normal in null embryos and in embryoid bodies of {alpha}1LG4–5-null embryonic stem cells. Yet, stem cells could not develop into polarized epiblast cells. Thus, {alpha}1LG4–5 provides vital signals for the conversion of stem cells to polarized epithelium.}}, author = {{Schéele, Susanne and Falk, Mats and Franzén, Ahnders and Ellin, Fredrik and Ferletta, Maria and Lonai, Peter and Andersson, Björn and Timpl, Rupert and Forsberg, Erik and Ekblom, Peter}}, issn = {{1091-6490}}, keywords = {{epiblast; epithelial polarity; stem cells; mouse development}}, language = {{eng}}, number = {{5}}, pages = {{1502--1506}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{Laminin {alpha}1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly.}}, url = {{http://dx.doi.org/10.1073/pnas.0405095102}}, doi = {{10.1073/pnas.0405095102}}, volume = {{102}}, year = {{2005}}, }