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Development of Humanized Ossicles : Bridging the Hematopoietic Gap

Dupard, Steven J. LU ; Grigoryan, Ani LU ; Farhat, Stephanie ; Coutu, Daniel L. and Bourgine, Paul E. LU (2020) In Trends in Molecular Medicine
Abstract

Ectopic 'humanized ossicles' (hOss) are miniaturized, engineered human bone organs in mice displaying a similar structure and function to native mouse bones. However, they are composed of human mesenchymal derived cells forming a humanized bone marrow niche. This in vivo reconstitution of human skeletal and hematopoietic compartments provides an opportunity to investigate the cellular and molecular processes involved in their establishment and functions in a human setting. However, current hOs strategies vary in their engineering methods and their downstream applications, undermining comprehensive exploitation of their potential. This review describes the specificities of the hOs models and highlights their potential and limits.... (More)

Ectopic 'humanized ossicles' (hOss) are miniaturized, engineered human bone organs in mice displaying a similar structure and function to native mouse bones. However, they are composed of human mesenchymal derived cells forming a humanized bone marrow niche. This in vivo reconstitution of human skeletal and hematopoietic compartments provides an opportunity to investigate the cellular and molecular processes involved in their establishment and functions in a human setting. However, current hOs strategies vary in their engineering methods and their downstream applications, undermining comprehensive exploitation of their potential. This review describes the specificities of the hOs models and highlights their potential and limits. Ultimately, we propose directions for the development of hOss as a technological platform for human hematopoietic studies.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
bone marrow niche, bone tissue engineering, hematopoiesis, hematopoietic stem cells, humanized ossicle, mesenchymal stromal cells
in
Trends in Molecular Medicine
publisher
Elsevier
external identifiers
  • pmid:32470383
  • scopus:85080905352
ISSN
1471-4914
DOI
10.1016/j.molmed.2020.01.016
language
English
LU publication?
yes
id
132dbd87-58fa-4acf-a207-a4c1bf99b0cb
date added to LUP
2020-03-20 16:45:05
date last changed
2020-10-27 02:14:04
@article{132dbd87-58fa-4acf-a207-a4c1bf99b0cb,
  abstract     = {<p>Ectopic 'humanized ossicles' (hOss) are miniaturized, engineered human bone organs in mice displaying a similar structure and function to native mouse bones. However, they are composed of human mesenchymal derived cells forming a humanized bone marrow niche. This in vivo reconstitution of human skeletal and hematopoietic compartments provides an opportunity to investigate the cellular and molecular processes involved in their establishment and functions in a human setting. However, current hOs strategies vary in their engineering methods and their downstream applications, undermining comprehensive exploitation of their potential. This review describes the specificities of the hOs models and highlights their potential and limits. Ultimately, we propose directions for the development of hOss as a technological platform for human hematopoietic studies.</p>},
  author       = {Dupard, Steven J. and Grigoryan, Ani and Farhat, Stephanie and Coutu, Daniel L. and Bourgine, Paul E.},
  issn         = {1471-4914},
  language     = {eng},
  month        = {03},
  publisher    = {Elsevier},
  series       = {Trends in Molecular Medicine},
  title        = {Development of Humanized Ossicles : Bridging the Hematopoietic Gap},
  url          = {http://dx.doi.org/10.1016/j.molmed.2020.01.016},
  doi          = {10.1016/j.molmed.2020.01.016},
  year         = {2020},
}