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Differential effects of glycoprotein B epitope-specific antibodies on human cytomegalovirus-induced cell–cell fusion

Gicklhorn, Dorothee; Eickmann, Markus; Meyer, Grit; Ohlin, Mats LU and Radsak, Klaus (2003) In Journal of General Virology 84(7). p.1859-1862
Abstract
Attachment of, and cell–cell fusion induced by, human cytomegalovirus were studied in the presence of neutralizing monospecific antibodies against antigenic domains 1 (AD-1) or 2 (AD-2) of glycoprotein B (gB, gpUL55). Efficient inhibition of the virion-mediated fusion event was consistently observed for the human AD-2-specific antibody as determined by a reporter gene activation assay based on permissive astrocytoma cells. In contrast, antibodies directed against the major neutralizing gB epitope AD-1 reduced fusion only by 20–60 %. Virus attachment via heparan sulfate was unaffected by the antibodies under the conditions used. Virus receptor binding as examined by heparin treatment of adsorbed virus was significantly reduced only if the... (More)
Attachment of, and cell–cell fusion induced by, human cytomegalovirus were studied in the presence of neutralizing monospecific antibodies against antigenic domains 1 (AD-1) or 2 (AD-2) of glycoprotein B (gB, gpUL55). Efficient inhibition of the virion-mediated fusion event was consistently observed for the human AD-2-specific antibody as determined by a reporter gene activation assay based on permissive astrocytoma cells. In contrast, antibodies directed against the major neutralizing gB epitope AD-1 reduced fusion only by 20–60 %. Virus attachment via heparan sulfate was unaffected by the antibodies under the conditions used. Virus receptor binding as examined by heparin treatment of adsorbed virus was significantly reduced only if the virus had been coated with the AD-2-specific antibody. Neutralization of virus infectivity by the AD-2-specific antibody thus seems most likely to result from interference with a receptor-binding event during initial virus–host cell interaction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
84
issue
7
pages
1859 - 1862
publisher
Society for General Microbiology
external identifiers
  • pmid:12810880
  • wos:000183937200023
  • scopus:0038150861
ISSN
1465-2099
DOI
10.1099/vir.0.19017-0
language
English
LU publication?
yes
id
2d2c1912-37bb-42ba-8359-df90413cad7f (old id 134286)
date added to LUP
2007-07-05 08:56:53
date last changed
2018-10-03 11:34:55
@article{2d2c1912-37bb-42ba-8359-df90413cad7f,
  abstract     = {Attachment of, and cell–cell fusion induced by, human cytomegalovirus were studied in the presence of neutralizing monospecific antibodies against antigenic domains 1 (AD-1) or 2 (AD-2) of glycoprotein B (gB, gpUL55). Efficient inhibition of the virion-mediated fusion event was consistently observed for the human AD-2-specific antibody as determined by a reporter gene activation assay based on permissive astrocytoma cells. In contrast, antibodies directed against the major neutralizing gB epitope AD-1 reduced fusion only by 20–60 %. Virus attachment via heparan sulfate was unaffected by the antibodies under the conditions used. Virus receptor binding as examined by heparin treatment of adsorbed virus was significantly reduced only if the virus had been coated with the AD-2-specific antibody. Neutralization of virus infectivity by the AD-2-specific antibody thus seems most likely to result from interference with a receptor-binding event during initial virus–host cell interaction.},
  author       = {Gicklhorn, Dorothee and Eickmann, Markus and Meyer, Grit and Ohlin, Mats and Radsak, Klaus},
  issn         = {1465-2099},
  language     = {eng},
  number       = {7},
  pages        = {1859--1862},
  publisher    = {Society for General Microbiology},
  series       = {Journal of General Virology},
  title        = {Differential effects of glycoprotein B epitope-specific antibodies on human cytomegalovirus-induced cell–cell fusion},
  url          = {http://dx.doi.org/10.1099/vir.0.19017-0},
  volume       = {84},
  year         = {2003},
}