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CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays

Högerkorp, Carl-Magnus LU ; Bilke, Sven LU ; Breslin, Thomas LU ; Ingvarsson, Sigurdur LU and Borrebaeck, Carl LU (2003) In Blood 101(6). p.2307-2313
Abstract
A number of studies have implicated a role for the cell surface glycoprotein CD44 in several biologic events, such as lymphopoiesis, homing, lymphocyte activation, and apoptosis. We have earlier reported that signaling via CD44 on naive B cells in addition to B-cell receptor (BCR) and CD40 engagement generated a germinal center-like phenotype. To further characterize the global role of CD44 in B differentiation, we examined the expression profile of human B cells cultured in vitro in the presence or absence of CD44 ligation, together with anti-immunoglobulin (anti-Ig) and anti-CD40 antibodies. The data sets derived from DNA microarrays were analyzed using a novel statistical analysis scheme created to retrieve the most likely expression... (More)
A number of studies have implicated a role for the cell surface glycoprotein CD44 in several biologic events, such as lymphopoiesis, homing, lymphocyte activation, and apoptosis. We have earlier reported that signaling via CD44 on naive B cells in addition to B-cell receptor (BCR) and CD40 engagement generated a germinal center-like phenotype. To further characterize the global role of CD44 in B differentiation, we examined the expression profile of human B cells cultured in vitro in the presence or absence of CD44 ligation, together with anti-immunoglobulin (anti-Ig) and anti-CD40 antibodies. The data sets derived from DNA microarrays were analyzed using a novel statistical analysis scheme created to retrieve the most likely expression pattern of CD44 ligation. Our results show that genes such as interleukin-6 (IL-6), IL-1alpha , and beta 2-adrenergic receptor (beta 2-AR) were specifically up-regulated by CD44 ligation, suggesting a novel role for CD44 in immunoregulation and inflammation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Blood
volume
101
issue
6
pages
2307 - 2313
publisher
American Society of Hematology
external identifiers
  • wos:000181432600036
  • pmid:12411303
  • scopus:0037443514
ISSN
1528-0020
DOI
language
English
LU publication?
yes
id
954a5148-bb44-44ce-b398-7fc65f645b4a (old id 134293)
date added to LUP
2007-07-05 11:03:08
date last changed
2018-06-17 03:58:13
@article{954a5148-bb44-44ce-b398-7fc65f645b4a,
  abstract     = {A number of studies have implicated a role for the cell surface glycoprotein CD44 in several biologic events, such as lymphopoiesis, homing, lymphocyte activation, and apoptosis. We have earlier reported that signaling via CD44 on naive B cells in addition to B-cell receptor (BCR) and CD40 engagement generated a germinal center-like phenotype. To further characterize the global role of CD44 in B differentiation, we examined the expression profile of human B cells cultured in vitro in the presence or absence of CD44 ligation, together with anti-immunoglobulin (anti-Ig) and anti-CD40 antibodies. The data sets derived from DNA microarrays were analyzed using a novel statistical analysis scheme created to retrieve the most likely expression pattern of CD44 ligation. Our results show that genes such as interleukin-6 (IL-6), IL-1alpha , and beta 2-adrenergic receptor (beta 2-AR) were specifically up-regulated by CD44 ligation, suggesting a novel role for CD44 in immunoregulation and inflammation.},
  author       = {Högerkorp, Carl-Magnus and Bilke, Sven and Breslin, Thomas and Ingvarsson, Sigurdur and Borrebaeck, Carl},
  issn         = {1528-0020},
  language     = {eng},
  number       = {6},
  pages        = {2307--2313},
  publisher    = {American Society of Hematology},
  series       = {Blood},
  title        = {CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays},
  url          = {http://dx.doi.org/},
  volume       = {101},
  year         = {2003},
}