Advanced

Analysis of Gas6 in Human Platelets and Plasma.

Balogh, Istvan LU ; Hafizi, Sassan LU ; Stenhoff, Jonas LU ; Hansson, Karin M LU and Dahlbäck, Björn LU (2005) In Arteriosclerosis, Thrombosis and Vascular Biology 25(6). p.1280-1286
Abstract
Objective - Gas6 is a member of the vitamin K-dependent protein family. Gas6- deficient mice were found to be resistant to thrombosis because of defective platelet function. Mouse Gas6 was demonstrated to be present in platelets and found to be involved in platelet aggregation. The aim of this study was to investigate the presence of Gas6 in human platelets and plasma and determine its role in platelet function. Methods and Results - The presence of Gas6 in human platelets and plasma was analyzed using sensitive immunologic methods. Mass spectrometry and ELISA were used to identify and quantify Gas6 in plasma. Gas6 was demonstrated to be present in human plasma, at a concentration determined to be 13 to 23 ng/mL (0.16 to 0.28 nM).... (More)
Objective - Gas6 is a member of the vitamin K-dependent protein family. Gas6- deficient mice were found to be resistant to thrombosis because of defective platelet function. Mouse Gas6 was demonstrated to be present in platelets and found to be involved in platelet aggregation. The aim of this study was to investigate the presence of Gas6 in human platelets and plasma and determine its role in platelet function. Methods and Results - The presence of Gas6 in human platelets and plasma was analyzed using sensitive immunologic methods. Mass spectrometry and ELISA were used to identify and quantify Gas6 in plasma. Gas6 was demonstrated to be present in human plasma, at a concentration determined to be 13 to 23 ng/mL (0.16 to 0.28 nM). Furthermore, plasma Gas6 levels were found to be lower in patients administered with warfarin. However, Gas6 was undetectable in human platelets. Conclusions - This is the first report to identify and quantify Gas6 in human plasma. However, Gas6 protein was not detected in human platelets, suggesting that any potential platelet-specific function could be because of Gas6 from the circulation. These findings open up new directions regarding the role of Gas6 in normal and pathophysiological situations such as inflammation, autoimmune disease, thrombosis and arteriosclerosis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
warfarin, vitamin K, Gas6, protein S, platelets, Gla
in
Arteriosclerosis, Thrombosis and Vascular Biology
volume
25
issue
6
pages
1280 - 1286
publisher
American Heart Association
external identifiers
  • wos:000229465000034
  • pmid:15790929
  • scopus:20144365611
ISSN
1524-4636
DOI
10.1161/01.ATV.0000163845.07146.48
language
English
LU publication?
yes
id
2edd8737-daaa-4fa4-b3f3-984c97a72a99 (old id 134768)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15790929&dopt=Abstract
date added to LUP
2007-07-11 15:40:51
date last changed
2017-09-24 03:37:06
@article{2edd8737-daaa-4fa4-b3f3-984c97a72a99,
  abstract     = {Objective - Gas6 is a member of the vitamin K-dependent protein family. Gas6- deficient mice were found to be resistant to thrombosis because of defective platelet function. Mouse Gas6 was demonstrated to be present in platelets and found to be involved in platelet aggregation. The aim of this study was to investigate the presence of Gas6 in human platelets and plasma and determine its role in platelet function. Methods and Results - The presence of Gas6 in human platelets and plasma was analyzed using sensitive immunologic methods. Mass spectrometry and ELISA were used to identify and quantify Gas6 in plasma. Gas6 was demonstrated to be present in human plasma, at a concentration determined to be 13 to 23 ng/mL (0.16 to 0.28 nM). Furthermore, plasma Gas6 levels were found to be lower in patients administered with warfarin. However, Gas6 was undetectable in human platelets. Conclusions - This is the first report to identify and quantify Gas6 in human plasma. However, Gas6 protein was not detected in human platelets, suggesting that any potential platelet-specific function could be because of Gas6 from the circulation. These findings open up new directions regarding the role of Gas6 in normal and pathophysiological situations such as inflammation, autoimmune disease, thrombosis and arteriosclerosis.},
  author       = {Balogh, Istvan and Hafizi, Sassan and Stenhoff, Jonas and Hansson, Karin M and Dahlbäck, Björn},
  issn         = {1524-4636},
  keyword      = {warfarin,vitamin K,Gas6,protein S,platelets,Gla},
  language     = {eng},
  number       = {6},
  pages        = {1280--1286},
  publisher    = {American Heart Association},
  series       = {Arteriosclerosis, Thrombosis and Vascular Biology},
  title        = {Analysis of Gas6 in Human Platelets and Plasma.},
  url          = {http://dx.doi.org/10.1161/01.ATV.0000163845.07146.48},
  volume       = {25},
  year         = {2005},
}