Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa
(2000) In Revista cubana de medicina tropical 52(1). p.15-20- Abstract
For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/134e603d-864b-49f5-ae35-5983f4f40aa8
- author
- Sarmiento Pérez, L LU ; Avalos Redón, I ; Ramos Valdés, Y ; Más Lago, P ; Bello Corredor, M ; Palomera Puentes, R and Barrios Olivera, J
- alternative title
- Rapid detection of Enterovirus by a direct method of polymerase chain reaction
- publishing date
- 2000-12-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Base Sequence, DNA, Viral/genetics, Enterovirus/genetics, Enterovirus Infections/diagnosis, Humans, Meningoencephalitis/diagnosis, Molecular Sequence Data, Polymerase Chain Reaction/methods, RNA, Viral/genetics, Sensitivity and Specificity
- in
- Revista cubana de medicina tropical
- volume
- 52
- issue
- 1
- pages
- 6 pages
- publisher
- Editorial Ciencias Medicas
- external identifiers
-
- scopus:0033758720
- pmid:11107888
- ISSN
- 0375-0760
- language
- Spanish
- LU publication?
- no
- id
- 134e603d-864b-49f5-ae35-5983f4f40aa8
- alternative location
- http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602000000100003
- date added to LUP
- 2019-07-08 15:15:13
- date last changed
- 2024-01-01 15:46:21
@article{134e603d-864b-49f5-ae35-5983f4f40aa8, abstract = {{<p>For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.</p>}}, author = {{Sarmiento Pérez, L and Avalos Redón, I and Ramos Valdés, Y and Más Lago, P and Bello Corredor, M and Palomera Puentes, R and Barrios Olivera, J}}, issn = {{0375-0760}}, keywords = {{Base Sequence; DNA, Viral/genetics; Enterovirus/genetics; Enterovirus Infections/diagnosis; Humans; Meningoencephalitis/diagnosis; Molecular Sequence Data; Polymerase Chain Reaction/methods; RNA, Viral/genetics; Sensitivity and Specificity}}, language = {{spa}}, month = {{12}}, number = {{1}}, pages = {{15--20}}, publisher = {{Editorial Ciencias Medicas}}, series = {{Revista cubana de medicina tropical}}, title = {{Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa}}, url = {{http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602000000100003}}, volume = {{52}}, year = {{2000}}, }