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Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa

Sarmiento Pérez, L LU ; Avalos Redón, I ; Ramos Valdés, Y ; Más Lago, P ; Bello Corredor, M ; Palomera Puentes, R and Barrios Olivera, J (2000) In Revista cubana de medicina tropical 52(1). p.15-20
Abstract

For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.

Please use this url to cite or link to this publication:
author
; ; ; ; ; and
alternative title
Rapid detection of Enterovirus by a direct method of polymerase chain reaction
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Base Sequence, DNA, Viral/genetics, Enterovirus/genetics, Enterovirus Infections/diagnosis, Humans, Meningoencephalitis/diagnosis, Molecular Sequence Data, Polymerase Chain Reaction/methods, RNA, Viral/genetics, Sensitivity and Specificity
in
Revista cubana de medicina tropical
volume
52
issue
1
pages
6 pages
publisher
Editorial Ciencias Medicas
external identifiers
  • pmid:11107888
  • scopus:0033758720
ISSN
0375-0760
language
Spanish
LU publication?
no
id
134e603d-864b-49f5-ae35-5983f4f40aa8
alternative location
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602000000100003
date added to LUP
2019-07-08 15:15:13
date last changed
2024-01-01 15:46:21
@article{134e603d-864b-49f5-ae35-5983f4f40aa8,
  abstract     = {{<p>For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.</p>}},
  author       = {{Sarmiento Pérez, L and Avalos Redón, I and Ramos Valdés, Y and Más Lago, P and Bello Corredor, M and Palomera Puentes, R and Barrios Olivera, J}},
  issn         = {{0375-0760}},
  keywords     = {{Base Sequence; DNA, Viral/genetics; Enterovirus/genetics; Enterovirus Infections/diagnosis; Humans; Meningoencephalitis/diagnosis; Molecular Sequence Data; Polymerase Chain Reaction/methods; RNA, Viral/genetics; Sensitivity and Specificity}},
  language     = {{spa}},
  month        = {{12}},
  number       = {{1}},
  pages        = {{15--20}},
  publisher    = {{Editorial Ciencias Medicas}},
  series       = {{Revista cubana de medicina tropical}},
  title        = {{Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa}},
  url          = {{http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602000000100003}},
  volume       = {{52}},
  year         = {{2000}},
}