Decreased glutathione transferase levels in rd1/rd1 mouse retina: Replenishment protects photoreceptors in retinal explants.
(2005) In Neuroscience 131(4). p.935-943- Abstract
- Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early... (More)
- Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early presence in ganglion cell layer after which staining became restricted to Muller cells (particularly in the endfeet) and horizontal cell fibers in both rd1/rd1 and +/+. Doublets of alpha-GST and mu-GST were detected by Western blot analysis. Densitometry of these bands indicated steady reduction of alpha-GST content in rd1/rd1 retina starting from the second postnatal week. When alpha-GST and mu-GST were added exogenously to rd1/rd1 explants, photoreceptor rescue was produced that was more prominent in PN2 than in PN7 explants and more effective by alpha-GST than mu-GST. We propose that alpha-GST neuroprotection is mediated by reduction of tissue oxidative stress. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/135168
- author
- Ahuja, Poonam LU ; Caffé, Romeo LU ; Ahuja, Sat pal LU ; Ekström, Per LU and van Veen, Theo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- retinal degeneration, culture, GST, neuroprotection
- in
- Neuroscience
- volume
- 131
- issue
- 4
- pages
- 935 - 943
- publisher
- Elsevier
- external identifiers
-
- pmid:15749346
- wos:000227792600015
- scopus:14644401785
- ISSN
- 1873-7544
- DOI
- 10.1016/j.neuroscience.2004.11.012
- language
- English
- LU publication?
- yes
- id
- 9bf99bd1-2e0f-4348-9ca8-cbb029dd98d6 (old id 135168)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15749346&dopt=Abstract
- date added to LUP
- 2016-04-01 12:19:48
- date last changed
- 2022-01-27 02:07:19
@article{9bf99bd1-2e0f-4348-9ca8-cbb029dd98d6, abstract = {{Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early presence in ganglion cell layer after which staining became restricted to Muller cells (particularly in the endfeet) and horizontal cell fibers in both rd1/rd1 and +/+. Doublets of alpha-GST and mu-GST were detected by Western blot analysis. Densitometry of these bands indicated steady reduction of alpha-GST content in rd1/rd1 retina starting from the second postnatal week. When alpha-GST and mu-GST were added exogenously to rd1/rd1 explants, photoreceptor rescue was produced that was more prominent in PN2 than in PN7 explants and more effective by alpha-GST than mu-GST. We propose that alpha-GST neuroprotection is mediated by reduction of tissue oxidative stress.}}, author = {{Ahuja, Poonam and Caffé, Romeo and Ahuja, Sat pal and Ekström, Per and van Veen, Theo}}, issn = {{1873-7544}}, keywords = {{retinal degeneration; culture; GST; neuroprotection}}, language = {{eng}}, number = {{4}}, pages = {{935--943}}, publisher = {{Elsevier}}, series = {{Neuroscience}}, title = {{Decreased glutathione transferase levels in rd1/rd1 mouse retina: Replenishment protects photoreceptors in retinal explants.}}, url = {{http://dx.doi.org/10.1016/j.neuroscience.2004.11.012}}, doi = {{10.1016/j.neuroscience.2004.11.012}}, volume = {{131}}, year = {{2005}}, }