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Decreased glutathione transferase levels in rd1/rd1 mouse retina: Replenishment protects photoreceptors in retinal explants.

Ahuja, Poonam LU ; Caffé, Romeo LU ; Ahuja, Sat pal LU ; Ekström, Per LU and van Veen, Theo LU (2005) In Neuroscience 131(4). p.935-943
Abstract
Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early... (More)
Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early presence in ganglion cell layer after which staining became restricted to Muller cells (particularly in the endfeet) and horizontal cell fibers in both rd1/rd1 and +/+. Doublets of alpha-GST and mu-GST were detected by Western blot analysis. Densitometry of these bands indicated steady reduction of alpha-GST content in rd1/rd1 retina starting from the second postnatal week. When alpha-GST and mu-GST were added exogenously to rd1/rd1 explants, photoreceptor rescue was produced that was more prominent in PN2 than in PN7 explants and more effective by alpha-GST than mu-GST. We propose that alpha-GST neuroprotection is mediated by reduction of tissue oxidative stress. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
retinal degeneration, culture, GST, neuroprotection
in
Neuroscience
volume
131
issue
4
pages
935 - 943
publisher
Elsevier
external identifiers
  • pmid:15749346
  • wos:000227792600015
  • scopus:14644401785
ISSN
1873-7544
DOI
10.1016/j.neuroscience.2004.11.012
language
English
LU publication?
yes
id
9bf99bd1-2e0f-4348-9ca8-cbb029dd98d6 (old id 135168)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15749346&dopt=Abstract
date added to LUP
2007-07-24 13:39:07
date last changed
2017-10-17 11:39:32
@article{9bf99bd1-2e0f-4348-9ca8-cbb029dd98d6,
  abstract     = {Currently much attention is focused on glutathione S transferase (GST)-induced suppression of apoptosis. The objective of our studies was therefore to see if GST isoenzymes rescue photoreceptors in retinal explants from rd1/rd1 mice, in which photoreceptors degenerate rapidly. Eyes from C3H rd1/rd1 and +/+ mice were collected at various time points between postnatal day (PN) 2 and PN28. Localization and content of alpha-GST and mu-GST was investigated by immunofluorescence and semi-quantitative Western blot analysis, respectively. In addition, PN2 and PN7 retinal explants were cultured till PN28, during which they were treated with 10 ng/ml alpha-GST or mu-GST. The spatiotemporal expression of both GST isoforms was closely similar: early presence in ganglion cell layer after which staining became restricted to Muller cells (particularly in the endfeet) and horizontal cell fibers in both rd1/rd1 and +/+. Doublets of alpha-GST and mu-GST were detected by Western blot analysis. Densitometry of these bands indicated steady reduction of alpha-GST content in rd1/rd1 retina starting from the second postnatal week. When alpha-GST and mu-GST were added exogenously to rd1/rd1 explants, photoreceptor rescue was produced that was more prominent in PN2 than in PN7 explants and more effective by alpha-GST than mu-GST. We propose that alpha-GST neuroprotection is mediated by reduction of tissue oxidative stress.},
  author       = {Ahuja, Poonam and Caffé, Romeo and Ahuja, Sat pal and Ekström, Per and van Veen, Theo},
  issn         = {1873-7544},
  keyword      = {retinal degeneration,culture,GST,neuroprotection},
  language     = {eng},
  number       = {4},
  pages        = {935--943},
  publisher    = {Elsevier},
  series       = {Neuroscience},
  title        = {Decreased glutathione transferase levels in rd1/rd1 mouse retina: Replenishment protects photoreceptors in retinal explants.},
  url          = {http://dx.doi.org/10.1016/j.neuroscience.2004.11.012},
  volume       = {131},
  year         = {2005},
}