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Colonisation and molecular diversity of arbuscular mycorrhizal fungi in the aquatic plants Littorella uniflora and Lobelia dortmanna in southern Sweden

Nielsen, K B; Kjoller, R; Olsson, Pål Axel LU ; Schweiger, P F; Andersen, F O and Rosendahl, S (2004) In Mycological Research1989-01-01+01:002010-01-01+01:00 108(6). p.616-625
Abstract
The colonisation intensity and composition of the mycorrhizal community in the aquatic plants Lobelia dortmanna and Littorella uniflora were studied. The mycorrhizal fungi were characterised by fungal specific nested PCR and sequencing using the 5'-end of the LSU rDNA as target. For this, primers for the clade of Acaulospora, the clade including Glomus mosseae and G. intraradices and the clade containing G. etunicatum and G. claroideum were used. The nested PCR products were screened for different sequence types using single stranded conformation polymorphism (SSCP) and representatives for each type were sequenced. A phylogenctic analysis of the sequences showed two phylotypes of Acaulospora, one phylotype within the clade of G.... (More)
The colonisation intensity and composition of the mycorrhizal community in the aquatic plants Lobelia dortmanna and Littorella uniflora were studied. The mycorrhizal fungi were characterised by fungal specific nested PCR and sequencing using the 5'-end of the LSU rDNA as target. For this, primers for the clade of Acaulospora, the clade including Glomus mosseae and G. intraradices and the clade containing G. etunicatum and G. claroideum were used. The nested PCR products were screened for different sequence types using single stranded conformation polymorphism (SSCP) and representatives for each type were sequenced. A phylogenctic analysis of the sequences showed two phylotypes of Acaulospora, one phylotype within the clade of G. etunicatum/G. claroideum and five within the G. mosseael G. intraradices clade. The colonisation intensity was comparable to that seen in typical grassland vegetation. The neutral lipid fatty acid 16: 1omega5 was seen to be indicative of mycorrhizal colonisation with concentrations up to 35 nmol mg(-1) root DW, which indicates that the fungi are active. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Mycological Research1989-01-01+01:002010-01-01+01:00
volume
108
issue
6
pages
616 - 625
publisher
Elsevier
external identifiers
  • wos:000222854300003
  • pmid:15323243
  • scopus:3142729569
ISSN
1469-8102
DOI
10.1017/S0953756204000073
language
English
LU publication?
yes
id
22ca5831-436f-468c-b5fd-21b1ed704ae4 (old id 135632)
date added to LUP
2007-06-29 13:42:25
date last changed
2017-11-28 14:56:31
@article{22ca5831-436f-468c-b5fd-21b1ed704ae4,
  abstract     = {The colonisation intensity and composition of the mycorrhizal community in the aquatic plants Lobelia dortmanna and Littorella uniflora were studied. The mycorrhizal fungi were characterised by fungal specific nested PCR and sequencing using the 5'-end of the LSU rDNA as target. For this, primers for the clade of Acaulospora, the clade including Glomus mosseae and G. intraradices and the clade containing G. etunicatum and G. claroideum were used. The nested PCR products were screened for different sequence types using single stranded conformation polymorphism (SSCP) and representatives for each type were sequenced. A phylogenctic analysis of the sequences showed two phylotypes of Acaulospora, one phylotype within the clade of G. etunicatum/G. claroideum and five within the G. mosseael G. intraradices clade. The colonisation intensity was comparable to that seen in typical grassland vegetation. The neutral lipid fatty acid 16: 1omega5 was seen to be indicative of mycorrhizal colonisation with concentrations up to 35 nmol mg(-1) root DW, which indicates that the fungi are active.},
  author       = {Nielsen, K B and Kjoller, R and Olsson, Pål Axel and Schweiger, P F and Andersen, F O and Rosendahl, S},
  issn         = {1469-8102},
  language     = {eng},
  number       = {6},
  pages        = {616--625},
  publisher    = {Elsevier},
  series       = {Mycological Research1989-01-01+01:002010-01-01+01:00},
  title        = {Colonisation and molecular diversity of arbuscular mycorrhizal fungi in the aquatic plants Littorella uniflora and Lobelia dortmanna in southern Sweden},
  url          = {http://dx.doi.org/10.1017/S0953756204000073},
  volume       = {108},
  year         = {2004},
}