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Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

Dickson, S; Schweiger, P; Smith, F A; Söderström, Bengt LU and Smith, S (2003) In Canadian Journal of Botany 81(5). p.457-463
Abstract
Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a... (More)
Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a single, radial intercellular hypha. These "paired" arbuscules often appeared to be at different developmental stages. Logistic regression and measurement of surface area indicated that there was a delay in initiation of the second arbuscule. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Canadian Journal of Botany
volume
81
issue
5
pages
457 - 463
publisher
Canadian Science Publishing, NRC Research Press
external identifiers
  • wos:000183913900006
  • scopus:0041846810
ISSN
0008-4026
DOI
10.1139/B03-037
language
English
LU publication?
yes
id
58b756df-554b-4aed-aaa9-915b0426e7fd (old id 135733)
alternative location
http://article.pubs.nrc-cnrc.gc.ca/ppv/RPViewDoc?_handler_=HandleInitialGet&journal=cjb&volume=81&calyLang=eng&articleFile=b03-037.pdf
date added to LUP
2007-06-29 09:30:35
date last changed
2018-09-23 03:42:47
@article{58b756df-554b-4aed-aaa9-915b0426e7fd,
  abstract     = {Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a single, radial intercellular hypha. These "paired" arbuscules often appeared to be at different developmental stages. Logistic regression and measurement of surface area indicated that there was a delay in initiation of the second arbuscule.},
  author       = {Dickson, S and Schweiger, P and Smith, F A and Söderström, Bengt and Smith, S},
  issn         = {0008-4026},
  language     = {eng},
  number       = {5},
  pages        = {457--463},
  publisher    = {Canadian Science Publishing, NRC Research Press},
  series       = {Canadian Journal of Botany},
  title        = {Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum},
  url          = {http://dx.doi.org/10.1139/B03-037},
  volume       = {81},
  year         = {2003},
}