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Deletion of a lectin gene does not affect the phenotype of the nematode-trapping fungus Arthrobotrys oligospora

Balogh, Johanna LU ; Tunlid, Anders LU and Rosén, Stefan LU (2003) In Fungal Genetics and Biology 39(2). p.128-135
Abstract
A number of filamentous fungi are known to produce high levels of saline-soluble and low-molecular-mass lectins. The function of these proteins are not clear but it has been proposed that they are involved in storage of nutrients, development, recognition of other organisms, and defense reactions. A gene encoding such a lectin (AOL) was deleted in the nematode-trapping fungus Arthrobotrys oligospora by homologous recombination. The deletion mutants did not express any hemagglutinating activity or protein cross-reacting with AOL antibodies. There were no significant differences between the DeltaAOL and wild-type strains in spore (conidia) germination, saprophytic growth, and pathogenicity. Furthermore, there was no significant difference in... (More)
A number of filamentous fungi are known to produce high levels of saline-soluble and low-molecular-mass lectins. The function of these proteins are not clear but it has been proposed that they are involved in storage of nutrients, development, recognition of other organisms, and defense reactions. A gene encoding such a lectin (AOL) was deleted in the nematode-trapping fungus Arthrobotrys oligospora by homologous recombination. The deletion mutants did not express any hemagglutinating activity or protein cross-reacting with AOL antibodies. There were no significant differences between the DeltaAOL and wild-type strains in spore (conidia) germination, saprophytic growth, and pathogenicity. Furthermore, there was no significant difference in the growth and reproduction of collembolan feeding on the various strains of A. oligospora. Thus either the previous proposed functions of AOL are not correct, or the fungus can compensate for the absence of the lectin by expressing other proteins with similar function(s) as AOL. (C) 2003 Published by Elsevier Science (USA). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Fungal Genetics and Biology
volume
39
issue
2
pages
128 - 135
publisher
Elsevier
external identifiers
  • pmid:12781671
  • wos:000183523800003
  • scopus:0038730856
ISSN
1087-1845
DOI
10.1016/S1087-1845(03)00023-9
language
English
LU publication?
yes
id
abb9f2d4-f098-4f86-8bba-38f709bc079c (old id 135751)
date added to LUP
2007-06-29 09:04:10
date last changed
2018-01-07 09:32:39
@article{abb9f2d4-f098-4f86-8bba-38f709bc079c,
  abstract     = {A number of filamentous fungi are known to produce high levels of saline-soluble and low-molecular-mass lectins. The function of these proteins are not clear but it has been proposed that they are involved in storage of nutrients, development, recognition of other organisms, and defense reactions. A gene encoding such a lectin (AOL) was deleted in the nematode-trapping fungus Arthrobotrys oligospora by homologous recombination. The deletion mutants did not express any hemagglutinating activity or protein cross-reacting with AOL antibodies. There were no significant differences between the DeltaAOL and wild-type strains in spore (conidia) germination, saprophytic growth, and pathogenicity. Furthermore, there was no significant difference in the growth and reproduction of collembolan feeding on the various strains of A. oligospora. Thus either the previous proposed functions of AOL are not correct, or the fungus can compensate for the absence of the lectin by expressing other proteins with similar function(s) as AOL. (C) 2003 Published by Elsevier Science (USA).},
  author       = {Balogh, Johanna and Tunlid, Anders and Rosén, Stefan},
  issn         = {1087-1845},
  language     = {eng},
  number       = {2},
  pages        = {128--135},
  publisher    = {Elsevier},
  series       = {Fungal Genetics and Biology},
  title        = {Deletion of a lectin gene does not affect the phenotype of the nematode-trapping fungus Arthrobotrys oligospora},
  url          = {http://dx.doi.org/10.1016/S1087-1845(03)00023-9},
  volume       = {39},
  year         = {2003},
}