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The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi

Bååth, Erland LU (2003) In Microbial Ecology 45(4). p.373-383
Abstract
The usefulness of measuring neutral lipid fatty acids (NLFAs) and phospholipid fatty acids (PLFAs) separately in order to interpret perturbation effects on soil and compost microorganisms has been studied. Initially the NLFA/PLFA ratios were studied in different soils. Low ratios were found for fatty acids common in bacteria, especially for cyclopropane fatty acids. Higher ratios were found for fatty acids common in eukaryotic organisms such as fungi (18:1(09 and 18:2omega6,9) or in saturated fatty acids, common to many types of organisms. Adding glucose to a forest soil increased the amounts of the fungal NLFAs 18:1omega9 and 18:2omega6,9 up to 60 and 10 times, respectively, after 10 days, followed by a gradual decrease. After 3 months... (More)
The usefulness of measuring neutral lipid fatty acids (NLFAs) and phospholipid fatty acids (PLFAs) separately in order to interpret perturbation effects on soil and compost microorganisms has been studied. Initially the NLFA/PLFA ratios were studied in different soils. Low ratios were found for fatty acids common in bacteria, especially for cyclopropane fatty acids. Higher ratios were found for fatty acids common in eukaryotic organisms such as fungi (18:1(09 and 18:2omega6,9) or in saturated fatty acids, common to many types of organisms. Adding glucose to a forest soil increased the amounts of the fungal NLFAs 18:1omega9 and 18:2omega6,9 up to 60 and 10 times, respectively, after 10 days, followed by a gradual decrease. After 3 months incubation, higher levels of these NLFAs were still found compared with the control samples. Adding glucose together with nitrogen (N) and phosphorus (P) resulted in no increase in NLFAs but a 10-fold increase in the PLFAs 18:1omega9 and 18:2omega6,9. Thus, the NLFA/PLFA ratios for these fatty acids were lower than in the no-addition control when glucose was added together with N and P, but higher when glucose was added alone, even 3 months after the addition. Adding N+P without glucose did not affect the NLFA/PLFA ratio for any fatty acid. Increasing NLFA/PLFA ratios for the fungal fatty acids were also found with time after the thermophilic phase in a compost, indicating increased availability of easily available carbon. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Ecology
volume
45
issue
4
pages
373 - 383
publisher
Springer
external identifiers
  • pmid:12704558
  • wos:000183289000006
  • scopus:0037667122
ISSN
1432-184X
DOI
10.1007/s00248-003-2002-y
language
English
LU publication?
yes
id
4d9d7287-d57e-42ac-bfdf-e2eb8f6cb5f8 (old id 135756)
date added to LUP
2007-06-29 09:07:59
date last changed
2018-02-18 04:23:33
@article{4d9d7287-d57e-42ac-bfdf-e2eb8f6cb5f8,
  abstract     = {The usefulness of measuring neutral lipid fatty acids (NLFAs) and phospholipid fatty acids (PLFAs) separately in order to interpret perturbation effects on soil and compost microorganisms has been studied. Initially the NLFA/PLFA ratios were studied in different soils. Low ratios were found for fatty acids common in bacteria, especially for cyclopropane fatty acids. Higher ratios were found for fatty acids common in eukaryotic organisms such as fungi (18:1(09 and 18:2omega6,9) or in saturated fatty acids, common to many types of organisms. Adding glucose to a forest soil increased the amounts of the fungal NLFAs 18:1omega9 and 18:2omega6,9 up to 60 and 10 times, respectively, after 10 days, followed by a gradual decrease. After 3 months incubation, higher levels of these NLFAs were still found compared with the control samples. Adding glucose together with nitrogen (N) and phosphorus (P) resulted in no increase in NLFAs but a 10-fold increase in the PLFAs 18:1omega9 and 18:2omega6,9. Thus, the NLFA/PLFA ratios for these fatty acids were lower than in the no-addition control when glucose was added together with N and P, but higher when glucose was added alone, even 3 months after the addition. Adding N+P without glucose did not affect the NLFA/PLFA ratio for any fatty acid. Increasing NLFA/PLFA ratios for the fungal fatty acids were also found with time after the thermophilic phase in a compost, indicating increased availability of easily available carbon.},
  author       = {Bååth, Erland},
  issn         = {1432-184X},
  language     = {eng},
  number       = {4},
  pages        = {373--383},
  publisher    = {Springer},
  series       = {Microbial Ecology},
  title        = {The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi},
  url          = {http://dx.doi.org/10.1007/s00248-003-2002-y},
  volume       = {45},
  year         = {2003},
}