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Effects of carbon substrate enrichment and DOC concentration on biodegradation of PAHs in soil

Bengtsson, Göran LU and Zerhouni, P (2003) In Journal of Applied Microbiology1997-01-01+01:00 94(4). p.608-617
Abstract
Aims: Two common reasons to explain slow environmental biodegradation of polycyclic aromatic hydrocarbons (PAHs), namely lack of appropriate carbon sources for microbial growth and limited bioavailability of PAHs, were tested in a laboratory bioassay using a creosote-contaminated soil. Methods and Results: The soil, containing a total of 8 mg g(-1) of 16 PAHs, was sieved and incubated in bottles for 45 days. The first explanation was tested by enrichment with the analogue anthracene and the non-analogue myristic acid, and both failed to stimulate degradation of all PAHs except anthracene. The second explanation was tested by addition of different concentrations of dissolved organic carbon (DOC), with effects depending on the DOC... (More)
Aims: Two common reasons to explain slow environmental biodegradation of polycyclic aromatic hydrocarbons (PAHs), namely lack of appropriate carbon sources for microbial growth and limited bioavailability of PAHs, were tested in a laboratory bioassay using a creosote-contaminated soil. Methods and Results: The soil, containing a total of 8 mg g(-1) of 16 PAHs, was sieved and incubated in bottles for 45 days. The first explanation was tested by enrichment with the analogue anthracene and the non-analogue myristic acid, and both failed to stimulate degradation of all PAHs except anthracene. The second explanation was tested by addition of different concentrations of dissolved organic carbon (DOC), with effects depending on the DOC concentration and the molecular size of the PAH. The degradation was enhanced from 10 to 35% for 12 PAHs when the soil was saturated. The degraded amounts of individual PAHs were proportional to their concentration in the soil. Conclusions: The slow in situ degradation of PAHs was enhanced by more than three times by adding water as a solvent. Addition of DOC facilitated the degradation of four- to six-ring PAHs. Significance and Impact of Study: Bioremediation of PAH-contaminated sites may be facilitated by creating water-saturated conditions but retarded by addition of other carbon substrates, such as analogue compounds. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Applied Microbiology1997-01-01+01:00
volume
94
issue
4
pages
608 - 617
publisher
Wiley-Blackwell
external identifiers
  • pmid:12631196
  • wos:000181549700009
  • scopus:0037271474
ISSN
1364-5072
DOI
10.1046/j.1365-2672.2003.01873.x
language
English
LU publication?
yes
id
da4b3726-c4df-452f-b87a-56a95c59ee20 (old id 135955)
date added to LUP
2007-06-27 08:30:58
date last changed
2018-09-30 03:34:20
@article{da4b3726-c4df-452f-b87a-56a95c59ee20,
  abstract     = {Aims: Two common reasons to explain slow environmental biodegradation of polycyclic aromatic hydrocarbons (PAHs), namely lack of appropriate carbon sources for microbial growth and limited bioavailability of PAHs, were tested in a laboratory bioassay using a creosote-contaminated soil. Methods and Results: The soil, containing a total of 8 mg g(-1) of 16 PAHs, was sieved and incubated in bottles for 45 days. The first explanation was tested by enrichment with the analogue anthracene and the non-analogue myristic acid, and both failed to stimulate degradation of all PAHs except anthracene. The second explanation was tested by addition of different concentrations of dissolved organic carbon (DOC), with effects depending on the DOC concentration and the molecular size of the PAH. The degradation was enhanced from 10 to 35% for 12 PAHs when the soil was saturated. The degraded amounts of individual PAHs were proportional to their concentration in the soil. Conclusions: The slow in situ degradation of PAHs was enhanced by more than three times by adding water as a solvent. Addition of DOC facilitated the degradation of four- to six-ring PAHs. Significance and Impact of Study: Bioremediation of PAH-contaminated sites may be facilitated by creating water-saturated conditions but retarded by addition of other carbon substrates, such as analogue compounds.},
  author       = {Bengtsson, Göran and Zerhouni, P},
  issn         = {1364-5072},
  language     = {eng},
  number       = {4},
  pages        = {608--617},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Applied Microbiology1997-01-01+01:00},
  title        = {Effects of carbon substrate enrichment and DOC concentration on biodegradation of PAHs in soil},
  url          = {http://dx.doi.org/10.1046/j.1365-2672.2003.01873.x},
  volume       = {94},
  year         = {2003},
}