Effect of active site-inactivated factor VIIa on ischaemia/reperfusion injury in a porcine flap model.
(2004) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 38(6). p.326-334- Abstract
- In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio,... (More)
- In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio, and accumulation of radiolabelled leucocytes, fibrinogen, and platelets were measured. There was no effect on tissue survival, but radiolabelled components in viable tissue were increased, though not significantly so. We conclude that FFR-rFVIIa did not prevent IR injury, indicating that tissue factor-mediated coagulation is not an important determinant of IR injury in this setting. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/136076
- author
- Söderström, Torbjörn LU ; Birk-Sørensen, Lene ; Holst-Jensen, Henrik ; Hedner, Ulla and Arnljots, Björn LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ischemia-reperfusion injury, coagulation, thrombosis prevention, free flaps
- in
- Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
- volume
- 38
- issue
- 6
- pages
- 326 - 334
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000225977200002
- pmid:15841799
- scopus:10644293901
- ISSN
- 1651-2073
- DOI
- 10.1080/02844310410016989
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200), Reconstructive Surgery (013240300), Surgery Research Unit (013242220)
- id
- 76c254cb-9bad-4ff4-bc32-6731443d5ae8 (old id 136076)
- date added to LUP
- 2016-04-01 16:54:00
- date last changed
- 2022-01-28 22:55:47
@article{76c254cb-9bad-4ff4-bc32-6731443d5ae8, abstract = {{In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio, and accumulation of radiolabelled leucocytes, fibrinogen, and platelets were measured. There was no effect on tissue survival, but radiolabelled components in viable tissue were increased, though not significantly so. We conclude that FFR-rFVIIa did not prevent IR injury, indicating that tissue factor-mediated coagulation is not an important determinant of IR injury in this setting.}}, author = {{Söderström, Torbjörn and Birk-Sørensen, Lene and Holst-Jensen, Henrik and Hedner, Ulla and Arnljots, Björn}}, issn = {{1651-2073}}, keywords = {{ischemia-reperfusion injury; coagulation; thrombosis prevention; free flaps}}, language = {{eng}}, number = {{6}}, pages = {{326--334}}, publisher = {{Taylor & Francis}}, series = {{Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery}}, title = {{Effect of active site-inactivated factor VIIa on ischaemia/reperfusion injury in a porcine flap model.}}, url = {{http://dx.doi.org/10.1080/02844310410016989}}, doi = {{10.1080/02844310410016989}}, volume = {{38}}, year = {{2004}}, }