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Effect of active site-inactivated factor VIIa on ischaemia/reperfusion injury in a porcine flap model.

Söderström, Torbjörn LU ; Birk-Sørensen, Lene; Holst-Jensen, Henrik; Hedner, Ulla and Arnljots, Björn LU (2004) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00 38(6). p.326-334
Abstract
In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio,... (More)
In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio, and accumulation of radiolabelled leucocytes, fibrinogen, and platelets were measured. There was no effect on tissue survival, but radiolabelled components in viable tissue were increased, though not significantly so. We conclude that FFR-rFVIIa did not prevent IR injury, indicating that tissue factor-mediated coagulation is not an important determinant of IR injury in this setting. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ischemia-reperfusion injury, coagulation, thrombosis prevention, free flaps
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00
volume
38
issue
6
pages
326 - 334
publisher
Taylor & Francis
external identifiers
  • wos:000225977200002
  • pmid:15841799
  • scopus:10644293901
ISSN
1651-2073
DOI
10.1080/02844310410016989
language
English
LU publication?
yes
id
76c254cb-9bad-4ff4-bc32-6731443d5ae8 (old id 136076)
date added to LUP
2007-07-30 14:10:49
date last changed
2017-11-30 08:23:49
@article{76c254cb-9bad-4ff4-bc32-6731443d5ae8,
  abstract     = {In free flap surgery, restored blood flow following a lengthy ischaemic period may lead to necrosis as a result of ischaemia/reperfusion (IR) injury. This injury comprises both proinflammatory and prothrombotic events, where the tissue factor/factor VIIa complex probably has a key role. Active site-inactivated factor VIIa (FFR-rFVIIa) exerts an antithrombotic effect by binding to tissue factor without initiating coagulation. In this study we have evaluated the potential protective effects of FFR-rFVIIa in IR injury. Bilateral musculocutaneous latissimus dorsi flaps in 16 pigs were made ischaemic for eight hours, then given 1 mg/kg/flap of FFR-rFVIIa or vehicle intra-arterially, and reperfused for 10 hours. The viable:necrotic tissue ratio, and accumulation of radiolabelled leucocytes, fibrinogen, and platelets were measured. There was no effect on tissue survival, but radiolabelled components in viable tissue were increased, though not significantly so. We conclude that FFR-rFVIIa did not prevent IR injury, indicating that tissue factor-mediated coagulation is not an important determinant of IR injury in this setting.},
  author       = {Söderström, Torbjörn and Birk-Sørensen, Lene and Holst-Jensen, Henrik and Hedner, Ulla and Arnljots, Björn},
  issn         = {1651-2073},
  keyword      = {ischemia-reperfusion injury,coagulation,thrombosis prevention,free flaps},
  language     = {eng},
  number       = {6},
  pages        = {326--334},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery1987-01-01+01:002010-01-01+01:00},
  title        = {Effect of active site-inactivated factor VIIa on ischaemia/reperfusion injury in a porcine flap model.},
  url          = {http://dx.doi.org/10.1080/02844310410016989},
  volume       = {38},
  year         = {2004},
}