Advanced

Effects of total parenteral nutrition on rat enteric nervous system, intestinal morphology, and motility.

Ekelund, Mikael LU ; Ekelund, Mats LU ; Qader, Saleem LU ; Hallén, Magnus LU and Ekblad, Eva LU (2005) In Journal of Surgical Research 124(2). p.187-193
Abstract
Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry,... (More)
Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry, the numbers of submucous (P < 0.05) and myenteric (P < 0.05) nerve cells were found to increase, expressed as numbers per unit length. The percentage of neurons expressing VIP, PACAP-27, NO-synthase, and galanin remained unchanged, however. By in situ hybridization the number of submucous neurons expressing neuropeptide Y-mRNA was found to decrease (P < 0.05); the other populations were unaltered. Morphometry revealed an increased submucosal thickness (P < 0.05), while intestinal circumference markedly decreased (P < 0.0001) in TPN-treated rats. In conclusion, TPN treatment resulted in reduced intestinal circumference leading to condensation of enteric neurons. No marked changes in neurotransmitter expression of the enteric neurons or in motor activity were noted. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
plasticity, motility, morphology, ENS, intestine, rat, TPN
in
Journal of Surgical Research
volume
124
issue
2
pages
187 - 193
publisher
Elsevier
external identifiers
  • pmid:15820247
  • wos:000228275800006
  • scopus:16244385635
ISSN
1095-8673
DOI
10.1016/j.jss.2004.10.008
language
English
LU publication?
yes
id
f9aa1556-7df9-4bf9-8496-be1feb0ea402 (old id 136281)
alternative location
http://www.sciencedirect.com/science/article/pii/S0022480404006365
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15820247&dopt=Abstract
date added to LUP
2007-07-11 14:53:15
date last changed
2017-01-01 06:47:34
@article{f9aa1556-7df9-4bf9-8496-be1feb0ea402,
  abstract     = {Total parenteral nutrition (TPN) is often crucial for patients not being able to feed enterally or having intestinal absorptive deficits. Enteral nutrition is, however, frequently regarded vital for maintaining functional and structural intestinal integrity. The aim of this study was to investigate possible effects of TPN on rat distal small intestine compared to enterally fed identically housed controls, regarding the enteric nervous system (ENS), motility in vitro, and morphology. This study shows that motor responses evoked by electrical stimulation or exposure to vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptide-27 (PACAP-27), and nitric oxide (NO) donor were unchanged. By using immunohistochemistry, the numbers of submucous (P &lt; 0.05) and myenteric (P &lt; 0.05) nerve cells were found to increase, expressed as numbers per unit length. The percentage of neurons expressing VIP, PACAP-27, NO-synthase, and galanin remained unchanged, however. By in situ hybridization the number of submucous neurons expressing neuropeptide Y-mRNA was found to decrease (P &lt; 0.05); the other populations were unaltered. Morphometry revealed an increased submucosal thickness (P &lt; 0.05), while intestinal circumference markedly decreased (P &lt; 0.0001) in TPN-treated rats. In conclusion, TPN treatment resulted in reduced intestinal circumference leading to condensation of enteric neurons. No marked changes in neurotransmitter expression of the enteric neurons or in motor activity were noted.},
  author       = {Ekelund, Mikael and Ekelund, Mats and Qader, Saleem and Hallén, Magnus and Ekblad, Eva},
  issn         = {1095-8673},
  keyword      = {plasticity,motility,morphology,ENS,intestine,rat,TPN},
  language     = {eng},
  number       = {2},
  pages        = {187--193},
  publisher    = {Elsevier},
  series       = {Journal of Surgical Research},
  title        = {Effects of total parenteral nutrition on rat enteric nervous system, intestinal morphology, and motility.},
  url          = {http://dx.doi.org/10.1016/j.jss.2004.10.008},
  volume       = {124},
  year         = {2005},
}