Prospectively isolated CD133/CD24-positive ependymal cells from the adult spinal cord and lateral ventricle wall differ in their long-term in vitro self-renewal and in vivo gene expression.
(2011) In GLIA 59(1). p.68-81- Abstract
- In contrast to ependymal cells located above the subventricular zone (SVZ) of the adult lateral ventricle wall (LVW), adult spinal cord (SC) ependymal cells possess certain neural stem cell characteristics. The molecular basis of this difference is unknown. In this study, antibodies against multiple cell surface markers were applied to isolate pure populations of SC and LVW ependymal cells, which allowed a direct comparison of their in vitro behavior and in vivo gene expression profile. Isolated CD133(+)/CD24(+)/CD45(-)/CD34(-) ependymal cells from the SC displayed in vitro self-renewal and differentiation capacity, whereas those from the LVW did not. SC ependymal cells showed a higher expression of several genes involved in cell division,... (More)
- In contrast to ependymal cells located above the subventricular zone (SVZ) of the adult lateral ventricle wall (LVW), adult spinal cord (SC) ependymal cells possess certain neural stem cell characteristics. The molecular basis of this difference is unknown. In this study, antibodies against multiple cell surface markers were applied to isolate pure populations of SC and LVW ependymal cells, which allowed a direct comparison of their in vitro behavior and in vivo gene expression profile. Isolated CD133(+)/CD24(+)/CD45(-)/CD34(-) ependymal cells from the SC displayed in vitro self-renewal and differentiation capacity, whereas those from the LVW did not. SC ependymal cells showed a higher expression of several genes involved in cell division, cell cycle regulation, and chromosome stability, which is consistent with a long-term self-renewal capacity, and shared certain transcripts with neural stem cells of the embryonic forebrain. They also expressed several retinoic acid (RA)-regulated genes and responded to RA exposure. LVW ependymal cells showed higher transcript levels of many genes regulated by transforming growth factor-β family members. Among them were Dlx2, Id2, Hey1, which together with Foxg1 could explain their potential to turn into neuroblasts under certain environmental conditions. © 2010 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1732360
- author
- Pfenninger, Cosima LU ; Steinhoff, Christine ; Hertwig, Falk LU and Nuber, Ulrike LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- GLIA
- volume
- 59
- issue
- 1
- pages
- 68 - 81
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000285209900007
- pmid:21046556
- scopus:78349285121
- pmid:21046556
- ISSN
- 1098-1136
- DOI
- 10.1002/glia.21077
- language
- English
- LU publication?
- yes
- id
- 1366cb17-c34a-4f3a-8e02-4c3da8e94ea0 (old id 1732360)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21046556?dopt=Abstract
- date added to LUP
- 2016-04-04 08:47:37
- date last changed
- 2022-07-24 19:57:05
@article{1366cb17-c34a-4f3a-8e02-4c3da8e94ea0,
abstract = {{In contrast to ependymal cells located above the subventricular zone (SVZ) of the adult lateral ventricle wall (LVW), adult spinal cord (SC) ependymal cells possess certain neural stem cell characteristics. The molecular basis of this difference is unknown. In this study, antibodies against multiple cell surface markers were applied to isolate pure populations of SC and LVW ependymal cells, which allowed a direct comparison of their in vitro behavior and in vivo gene expression profile. Isolated CD133(+)/CD24(+)/CD45(-)/CD34(-) ependymal cells from the SC displayed in vitro self-renewal and differentiation capacity, whereas those from the LVW did not. SC ependymal cells showed a higher expression of several genes involved in cell division, cell cycle regulation, and chromosome stability, which is consistent with a long-term self-renewal capacity, and shared certain transcripts with neural stem cells of the embryonic forebrain. They also expressed several retinoic acid (RA)-regulated genes and responded to RA exposure. LVW ependymal cells showed higher transcript levels of many genes regulated by transforming growth factor-β family members. Among them were Dlx2, Id2, Hey1, which together with Foxg1 could explain their potential to turn into neuroblasts under certain environmental conditions. © 2010 Wiley-Liss, Inc.}},
author = {{Pfenninger, Cosima and Steinhoff, Christine and Hertwig, Falk and Nuber, Ulrike}},
issn = {{1098-1136}},
language = {{eng}},
number = {{1}},
pages = {{68--81}},
publisher = {{John Wiley & Sons Inc.}},
series = {{GLIA}},
title = {{Prospectively isolated CD133/CD24-positive ependymal cells from the adult spinal cord and lateral ventricle wall differ in their long-term in vitro self-renewal and in vivo gene expression.}},
url = {{http://dx.doi.org/10.1002/glia.21077}},
doi = {{10.1002/glia.21077}},
volume = {{59}},
year = {{2011}},
}