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Dopamine neuron precursors within the developing human mesencephalon show radial glial characteristics.

Hebsgaard, Josephine LU ; Nelander Wahlestedt, Jenny LU ; Sabelström, Hanna LU ; Jönsson, Marie LU ; Stott, Simon and Parmar, Malin LU (2009) In GLIA 57. p.1648-1659
Abstract
Specification and differentiation of neural precursors into dopaminergic neurons within the ventral mesencephalon has been subject to much attention due to the implication of dopaminergic neurons in Parkinson's disease and the perspective of generating sources of therapeutically active cells to be used for cell replacement therapy for the disease. However, despite intensive research efforts, little is known about the characteristics of the dopamine neuron progenitors in human. We show that the dopamine neuron determinant LMX1a is expressed in the diencephalic and mesencephalic dopaminergic neuron domains during human development. Within the mesencephalon, LMX1a is expressed in the dopaminergic neurons and their progenitors located in the... (More)
Specification and differentiation of neural precursors into dopaminergic neurons within the ventral mesencephalon has been subject to much attention due to the implication of dopaminergic neurons in Parkinson's disease and the perspective of generating sources of therapeutically active cells to be used for cell replacement therapy for the disease. However, despite intensive research efforts, little is known about the characteristics of the dopamine neuron progenitors in human. We show that the dopamine neuron determinant LMX1a is expressed in the diencephalic and mesencephalic dopaminergic neuron domains during human development. Within the mesencephalon, LMX1a is expressed in the dopaminergic neurons and their progenitors located in the ventricular zone of the floor plate region. Furthermore, the neural progenitors in the developing human ventral mesencephalon have a radial morphology and express the radial glial markers Vimentin and BLBP. These radial glia are mitotic and act as precursors for the dopaminergic neurons. Finally, we show that progenitors isolated from the human ventral mesencephalon maintain their radial glial characteristics and neurogenic capacity after expansion in vitro, making them a promising future source of cells to be used in cell replacement therapy for Parkinson's disease. (c) 2009 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
GLIA
volume
57
pages
1648 - 1659
publisher
John Wiley & Sons
external identifiers
  • wos:000270934900007
  • pmid:19330857
  • scopus:70449625586
ISSN
1098-1136
DOI
10.1002/glia.20877
language
English
LU publication?
yes
id
97ae8584-6096-44bd-a53a-74c34da1f60e (old id 1367379)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19330857?dopt=Abstract
date added to LUP
2009-04-06 11:55:18
date last changed
2017-01-01 07:31:00
@article{97ae8584-6096-44bd-a53a-74c34da1f60e,
  abstract     = {Specification and differentiation of neural precursors into dopaminergic neurons within the ventral mesencephalon has been subject to much attention due to the implication of dopaminergic neurons in Parkinson's disease and the perspective of generating sources of therapeutically active cells to be used for cell replacement therapy for the disease. However, despite intensive research efforts, little is known about the characteristics of the dopamine neuron progenitors in human. We show that the dopamine neuron determinant LMX1a is expressed in the diencephalic and mesencephalic dopaminergic neuron domains during human development. Within the mesencephalon, LMX1a is expressed in the dopaminergic neurons and their progenitors located in the ventricular zone of the floor plate region. Furthermore, the neural progenitors in the developing human ventral mesencephalon have a radial morphology and express the radial glial markers Vimentin and BLBP. These radial glia are mitotic and act as precursors for the dopaminergic neurons. Finally, we show that progenitors isolated from the human ventral mesencephalon maintain their radial glial characteristics and neurogenic capacity after expansion in vitro, making them a promising future source of cells to be used in cell replacement therapy for Parkinson's disease. (c) 2009 Wiley-Liss, Inc.},
  author       = {Hebsgaard, Josephine and Nelander Wahlestedt, Jenny and Sabelström, Hanna and Jönsson, Marie and Stott, Simon and Parmar, Malin},
  issn         = {1098-1136},
  language     = {eng},
  pages        = {1648--1659},
  publisher    = {John Wiley & Sons},
  series       = {GLIA},
  title        = {Dopamine neuron precursors within the developing human mesencephalon show radial glial characteristics.},
  url          = {http://dx.doi.org/10.1002/glia.20877},
  volume       = {57},
  year         = {2009},
}