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Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer.

Yoshimatsu, Keiichi LU ; LeJeune, Jason; Spivak, David A and Ye, Lei LU (2009) In Analyst 134(4). p.719-724
Abstract
A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
134
issue
4
pages
719 - 724
publisher
Royal Society of Chemistry
external identifiers
  • wos:000264482300012
  • pmid:19305921
  • scopus:67149089150
ISSN
1364-5528
DOI
10.1039/b814967d
language
English
LU publication?
yes
id
f3e41eb5-b909-4f87-a2f4-b283da83a033 (old id 1367569)
date added to LUP
2009-04-07 08:08:53
date last changed
2017-08-27 04:55:08
@article{f3e41eb5-b909-4f87-a2f4-b283da83a033,
  abstract     = {A single bi-functional monomer, N,O-bismethacryloyl ethanolamine (NOBE), was used in precipitation polymerization system to synthesize molecularly imprinted polymer (MIP) microspheres. Highly specific binding sites were obtained for N-terminal protected neuropeptides, Boc-Leu-enkephalin and Pyr-Leu-enkephalin. The use of NOBE allowed binding sites to be formed in polymer microspheres that are able to recognize target peptides through the consensus C-terminal sequence. The interesting molecular binding results suggest a new approach for peptide analysis combining in situ chemical modification with MIP recognition under non-aqueous conditions.},
  author       = {Yoshimatsu, Keiichi and LeJeune, Jason and Spivak, David A and Ye, Lei},
  issn         = {1364-5528},
  language     = {eng},
  number       = {4},
  pages        = {719--724},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Peptide-imprinted polymer microspheres prepared by precipitation polymerization using a single bi-functional monomer.},
  url          = {http://dx.doi.org/10.1039/b814967d},
  volume       = {134},
  year         = {2009},
}