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CD133+ and nestin+ tumor-initiating cells dominate in N29 and N32 experimental gliomas.

Bexell, Daniel LU ; Gunnarsson, Salina LU ; Siesjö, Peter LU ; Bengzon, Johan and Darabi, Anna LU (2009) In International Journal of Cancer 125(1). p.15-22
Abstract
The current study was designed to critically evaluate the notion that cancer stem cell (CSC)-like cells constitute a subpopulation of cells within experimental gliomas. Virtually all cells within the N29 and N32 rat glioma models homogenously expressed CD133, the stem/progenitor marker nestin as well as the neural lineage markers glial fibrillary acidic protein, betaIII-tubulin, and CNPase in vitro. The phenotype was largely retained on exposure to conditions promoting differentiation in vitro and after intracranial implantation of tumor cells into syngeneic hosts. Unsorted adherently grown cells displayed very high clonogenicity in vitro and robust tumorigenicity in vivo. Single N29 and N32 tumor cells invariably formed clones in vitro,... (More)
The current study was designed to critically evaluate the notion that cancer stem cell (CSC)-like cells constitute a subpopulation of cells within experimental gliomas. Virtually all cells within the N29 and N32 rat glioma models homogenously expressed CD133, the stem/progenitor marker nestin as well as the neural lineage markers glial fibrillary acidic protein, betaIII-tubulin, and CNPase in vitro. The phenotype was largely retained on exposure to conditions promoting differentiation in vitro and after intracranial implantation of tumor cells into syngeneic hosts. Unsorted adherently grown cells displayed very high clonogenicity in vitro and robust tumorigenicity in vivo. Single N29 and N32 tumor cells invariably formed clones in vitro, and intracerebral inoculation of as few as 10 adherently growing N29 and N32 tumor cells, respectively, gave rise to a tumor. These results provide an alternative view on CSC-like cells in glioma models: sphere-formation is not a prerequisite for accumulation of tumorigenic cells, and CSC-like cells do not reside within a rare subpopulation of cells in these glioma models. N29 and N32 gliomas may accordingly be used for the development of treatment strategies directed specifically against a practically pure population of brain tumor-initiating CSC-like cells. (c) 2009 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glioma, cancer stem cell, rat, tumor, CD133, nestin
in
International Journal of Cancer
volume
125
issue
1
pages
15 - 22
publisher
John Wiley & Sons
external identifiers
  • wos:000266569200003
  • pmid:19291792
  • scopus:66149130683
ISSN
0020-7136
DOI
10.1002/ijc.24306
language
English
LU publication?
yes
id
0f9c65a5-b6bc-43d7-a90f-7503242c65e5 (old id 1367723)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19291792?dopt=Abstract
date added to LUP
2009-04-07 15:41:53
date last changed
2017-10-08 03:34:54
@article{0f9c65a5-b6bc-43d7-a90f-7503242c65e5,
  abstract     = {The current study was designed to critically evaluate the notion that cancer stem cell (CSC)-like cells constitute a subpopulation of cells within experimental gliomas. Virtually all cells within the N29 and N32 rat glioma models homogenously expressed CD133, the stem/progenitor marker nestin as well as the neural lineage markers glial fibrillary acidic protein, betaIII-tubulin, and CNPase in vitro. The phenotype was largely retained on exposure to conditions promoting differentiation in vitro and after intracranial implantation of tumor cells into syngeneic hosts. Unsorted adherently grown cells displayed very high clonogenicity in vitro and robust tumorigenicity in vivo. Single N29 and N32 tumor cells invariably formed clones in vitro, and intracerebral inoculation of as few as 10 adherently growing N29 and N32 tumor cells, respectively, gave rise to a tumor. These results provide an alternative view on CSC-like cells in glioma models: sphere-formation is not a prerequisite for accumulation of tumorigenic cells, and CSC-like cells do not reside within a rare subpopulation of cells in these glioma models. N29 and N32 gliomas may accordingly be used for the development of treatment strategies directed specifically against a practically pure population of brain tumor-initiating CSC-like cells. (c) 2009 Wiley-Liss, Inc.},
  author       = {Bexell, Daniel and Gunnarsson, Salina and Siesjö, Peter and Bengzon, Johan and Darabi, Anna},
  issn         = {0020-7136},
  keyword      = {glioma,cancer stem cell,rat,tumor,CD133,nestin},
  language     = {eng},
  number       = {1},
  pages        = {15--22},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {CD133+ and nestin+ tumor-initiating cells dominate in N29 and N32 experimental gliomas.},
  url          = {http://dx.doi.org/10.1002/ijc.24306},
  volume       = {125},
  year         = {2009},
}