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Individual Oocysts Of Isospora (Apicomplexa: Coccidia) Parasites From Avian Feces: From Photo To Sequence

Dolnik, Olga V.; Palinauskas, Vaidas and Bensch, Staffan LU (2009) In Journal of Parasitology 95(1). p.169-174
Abstract
Numerous microscopic studies of coccidian oocysts from avian feces have become the basis for species identification. In contrast, molecular studies of wild birds' Coccidia are still in their infancy and are mostly based on DNA extracted from the blood stages of these parasites. Linking microscopic and molecular data requires a method that reliably extracts DNA from single oocysts with parallel detailed morphological examination of the same cell. We offer a thorough manual of isolating, photographing, and trapping single oocysts from avian feces, followed by extraction of parasite DNA and amplification of mitochondrial DNA from the same cells. In 39 single oocysts from 6 wild blackcaps, we combined microscopic studies of individual cells... (More)
Numerous microscopic studies of coccidian oocysts from avian feces have become the basis for species identification. In contrast, molecular studies of wild birds' Coccidia are still in their infancy and are mostly based on DNA extracted from the blood stages of these parasites. Linking microscopic and molecular data requires a method that reliably extracts DNA from single oocysts with parallel detailed morphological examination of the same cell. We offer a thorough manual of isolating, photographing, and trapping single oocysts from avian feces, followed by extraction of parasite DNA and amplification of mitochondrial DNA from the same cells. In 39 single oocysts from 6 wild blackcaps, we combined microscopic studies of individual cells with studies on their mitochondrial haplotype. In 72% of the single oocysts sampled, we detected unambiguous sequences. From feces and blood of investigated birds, we obtained 6 different haplotypes of Isospora sp. (iSATI-iSAT 6), finding both the same haplotype in different host individuals and various haplotypes in the same host individual. Our described methodology enables linking the huge amount of morphological data with innovative gene analysis. This method expands the scope of genetic studies conducted on Isospora species, including routine molecular analysis of single oocysts isolated from fecal samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Parasitology
volume
95
issue
1
pages
169 - 174
publisher
American Society of Parasitologists
external identifiers
  • wos:000263883100027
  • scopus:64549124310
ISSN
0022-3395
language
English
LU publication?
yes
id
9179ef2a-a1d5-4402-8f7b-dac3d06a5a8c (old id 1370676)
date added to LUP
2009-05-08 14:42:39
date last changed
2017-06-25 04:03:35
@article{9179ef2a-a1d5-4402-8f7b-dac3d06a5a8c,
  abstract     = {Numerous microscopic studies of coccidian oocysts from avian feces have become the basis for species identification. In contrast, molecular studies of wild birds' Coccidia are still in their infancy and are mostly based on DNA extracted from the blood stages of these parasites. Linking microscopic and molecular data requires a method that reliably extracts DNA from single oocysts with parallel detailed morphological examination of the same cell. We offer a thorough manual of isolating, photographing, and trapping single oocysts from avian feces, followed by extraction of parasite DNA and amplification of mitochondrial DNA from the same cells. In 39 single oocysts from 6 wild blackcaps, we combined microscopic studies of individual cells with studies on their mitochondrial haplotype. In 72% of the single oocysts sampled, we detected unambiguous sequences. From feces and blood of investigated birds, we obtained 6 different haplotypes of Isospora sp. (iSATI-iSAT 6), finding both the same haplotype in different host individuals and various haplotypes in the same host individual. Our described methodology enables linking the huge amount of morphological data with innovative gene analysis. This method expands the scope of genetic studies conducted on Isospora species, including routine molecular analysis of single oocysts isolated from fecal samples.},
  author       = {Dolnik, Olga V. and Palinauskas, Vaidas and Bensch, Staffan},
  issn         = {0022-3395},
  language     = {eng},
  number       = {1},
  pages        = {169--174},
  publisher    = {American Society of Parasitologists},
  series       = {Journal of Parasitology},
  title        = {Individual Oocysts Of Isospora (Apicomplexa: Coccidia) Parasites From Avian Feces: From Photo To Sequence},
  volume       = {95},
  year         = {2009},
}