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Glucose sensors with increased sensitivity based on composite gels containing immobilized boronic acid

Hajizadeh, Solmaz; Ivanov, Alexander LU ; Jahanshahi, Mohsen; Sanati, Mohamad H.; Zhuravleva, Natalia V.; Mikhalovska, Lyubov I. and Galaev, Igor LU (2008) In Reactive & Functional Polymers 68(12). p.1625-1635
Abstract
Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a... (More)
Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a 10-fold higher glucose sensitivity compared with the colorless NAAPBA-AAm gel. The equilibrium dissociation constants of soluble ARS-PVP and ARS-NAAPBA-containing copolymer complexes at pH 7.3 were found to be 1.4 and 0.44 mu M, respectively. The low dissociation constants enabled the preparation of the sensor with increased operational stability of at least two months allowing more than 20 assays of with no loss in the sensitivity or dye leakage. (C) 2008 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Composite hydrogel, Phenylboronic acid, Smart polymers, Optical response
in
Reactive & Functional Polymers
volume
68
issue
12
pages
1625 - 1635
publisher
Elsevier
external identifiers
  • wos:000261872700004
  • scopus:55949123609
ISSN
1873-166X
DOI
10.1016/j.reactfunctpolym.2008.09.006
language
English
LU publication?
yes
id
5dd2484c-1030-4a52-b59e-1187bb8ba971 (old id 1376364)
date added to LUP
2009-04-24 12:00:34
date last changed
2017-07-30 03:32:22
@article{5dd2484c-1030-4a52-b59e-1187bb8ba971,
  abstract     = {Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a 10-fold higher glucose sensitivity compared with the colorless NAAPBA-AAm gel. The equilibrium dissociation constants of soluble ARS-PVP and ARS-NAAPBA-containing copolymer complexes at pH 7.3 were found to be 1.4 and 0.44 mu M, respectively. The low dissociation constants enabled the preparation of the sensor with increased operational stability of at least two months allowing more than 20 assays of with no loss in the sensitivity or dye leakage. (C) 2008 Elsevier Ltd. All rights reserved.},
  author       = {Hajizadeh, Solmaz and Ivanov, Alexander and Jahanshahi, Mohsen and Sanati, Mohamad H. and Zhuravleva, Natalia V. and Mikhalovska, Lyubov I. and Galaev, Igor},
  issn         = {1873-166X},
  keyword      = {Composite hydrogel,Phenylboronic acid,Smart polymers,Optical response},
  language     = {eng},
  number       = {12},
  pages        = {1625--1635},
  publisher    = {Elsevier},
  series       = {Reactive & Functional Polymers},
  title        = {Glucose sensors with increased sensitivity based on composite gels containing immobilized boronic acid},
  url          = {http://dx.doi.org/10.1016/j.reactfunctpolym.2008.09.006},
  volume       = {68},
  year         = {2008},
}