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Ki67 and p53 immunohistochemistry reduces interobserver variation in assessment of Barrett's oesophagus.

Lörinc, Ester LU ; Jakobsson, B; Landberg, Göran LU and Veress, Bela LU (2005) In Histopathology 46(6). p.642-648
Abstract
Aims: To devise clinically applicable methods for assessing p53 and Ki67 immunohistochemical (IHC) reactivity in Barrett's oesophagus (BE) and to compare the interobserver agreement between these methods and routine haematoxylin and eosin (H&E) evaluation.



Methods and results: One hundred and fifteen biopsies diagnosed as BE, selected from the files of the University Hospital MAS, Malmö, were re-evaluated for dysplasia by three pathologists. For IHC analysis areas with the most prominent positivity were evaluated. The mean of p53+ epithelial nuclei/high-power field (HPF) was obtained by counting between 1 and 5 HPFs/biopsy. A proliferation quotient (PQ) was obtained by dividing the number of Ki67+ epithelial nuclei... (More)
Aims: To devise clinically applicable methods for assessing p53 and Ki67 immunohistochemical (IHC) reactivity in Barrett's oesophagus (BE) and to compare the interobserver agreement between these methods and routine haematoxylin and eosin (H&E) evaluation.



Methods and results: One hundred and fifteen biopsies diagnosed as BE, selected from the files of the University Hospital MAS, Malmö, were re-evaluated for dysplasia by three pathologists. For IHC analysis areas with the most prominent positivity were evaluated. The mean of p53+ epithelial nuclei/high-power field (HPF) was obtained by counting between 1 and 5 HPFs/biopsy. A proliferation quotient (PQ) was obtained by dividing the number of Ki67+ epithelial nuclei in the upper half by the lower half of the mucosa, using two HPFs. Mean κ values were 0.24, 0.71 and 0.52 for H&E, p53 and Ki67 evaluations, respectively. There was a correlation between increasing severity of dysplasia, IHC measurable overexpression of p53 and shift of the mucosal proliferation zone towards the surface, measured as PQ.



Conclusions: The described methods for p53 and Ki67 evaluation are more reproducible than routine H&E evaluation of BE. Furthermore, the IHC methods correlate with the severity of dysplasia and are useful supplementary prognostic markers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Histopathology
volume
46
issue
6
pages
642 - 648
publisher
Wiley-Blackwell
external identifiers
  • wos:000229780800005
  • pmid:15910595
  • scopus:20644458561
ISSN
0309-0167
DOI
10.1111/j.1365-2559.2005.02139.x
language
English
LU publication?
yes
id
aceba039-036e-47b6-ac31-3d1280eeff77 (old id 137870)
date added to LUP
2007-07-26 08:10:07
date last changed
2017-11-05 03:47:11
@article{aceba039-036e-47b6-ac31-3d1280eeff77,
  abstract     = {Aims: To devise clinically applicable methods for assessing p53 and Ki67 immunohistochemical (IHC) reactivity in Barrett's oesophagus (BE) and to compare the interobserver agreement between these methods and routine haematoxylin and eosin (H&amp;E) evaluation.<br/><br>
<br/><br>
Methods and results: One hundred and fifteen biopsies diagnosed as BE, selected from the files of the University Hospital MAS, Malmö, were re-evaluated for dysplasia by three pathologists. For IHC analysis areas with the most prominent positivity were evaluated. The mean of p53+ epithelial nuclei/high-power field (HPF) was obtained by counting between 1 and 5 HPFs/biopsy. A proliferation quotient (PQ) was obtained by dividing the number of Ki67+ epithelial nuclei in the upper half by the lower half of the mucosa, using two HPFs. Mean κ values were 0.24, 0.71 and 0.52 for H&amp;E, p53 and Ki67 evaluations, respectively. There was a correlation between increasing severity of dysplasia, IHC measurable overexpression of p53 and shift of the mucosal proliferation zone towards the surface, measured as PQ.<br/><br>
<br/><br>
Conclusions: The described methods for p53 and Ki67 evaluation are more reproducible than routine H&amp;E evaluation of BE. Furthermore, the IHC methods correlate with the severity of dysplasia and are useful supplementary prognostic markers.},
  author       = {Lörinc, Ester and Jakobsson, B and Landberg, Göran and Veress, Bela},
  issn         = {0309-0167},
  language     = {eng},
  number       = {6},
  pages        = {642--648},
  publisher    = {Wiley-Blackwell},
  series       = {Histopathology},
  title        = {Ki67 and p53 immunohistochemistry reduces interobserver variation in assessment of Barrett's oesophagus.},
  url          = {http://dx.doi.org/10.1111/j.1365-2559.2005.02139.x},
  volume       = {46},
  year         = {2005},
}