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Characterization of the microbial community in indoor environments by chemical marker analysis: an update and critical evaluation.

Sebastian, Aleksandra LU ; Szponar, Bogumila LU and Larsson, Lennart LU (2005) In Indoor Air 15 Suppl 9(9). p.20-26
Abstract
We published recently an integrated procedure for applying chemical marker analysis to characterize the microbiology of indoor environments comprising a scheme for extraction and analysis of markers of endotoxin, peptidoglycan/bacterial biomass, and fungal biomass. In the present paper, we report some significant improvements and also new possibilities of the described approach. We found that while 3-hydroxy fatty acids (3-OH FAs) of 10-14 carbon chain lengths are useful endotoxin markers, longer 3-OH FAs (i.e. with 16 carbon atoms and more) may rather serve as markers of Actinobacteria. We introduced C-13-labeled 3-hydroxytridecanoic acid, from labeled Pectinatus cerevisiiphilus, as an internal standard to improve quantification of the... (More)
We published recently an integrated procedure for applying chemical marker analysis to characterize the microbiology of indoor environments comprising a scheme for extraction and analysis of markers of endotoxin, peptidoglycan/bacterial biomass, and fungal biomass. In the present paper, we report some significant improvements and also new possibilities of the described approach. We found that while 3-hydroxy fatty acids (3-OH FAs) of 10-14 carbon chain lengths are useful endotoxin markers, longer 3-OH FAs (i.e. with 16 carbon atoms and more) may rather serve as markers of Actinobacteria. We introduced C-13-labeled 3-hydroxytridecanoic acid, from labeled Pectinatus cerevisiiphilus, as an internal standard to improve quantification of the 3-OH FAs in the gas chromatography-mass spectrometry analysis. Finally, in experiments aiming to identify a suitable method for collection of house dust for chemical marker analysis, we found that the marker compositions of dusts sedimented on plexiglass plates that were spatially well-distributed in a studied room at different heights above floor level, were undistinguishable. This type of sampling thus appears to be well suited for use, e.g. in epidemiological studies. In summary, the presented work describes important new capabilities of chemical marker analysis in defining human exposure to microorganisms in indoor environments. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
indoor air, muramic acid, chemical markers, microbiology, ergosterol, endotoxin
in
Indoor Air
volume
15 Suppl 9
issue
9
pages
20 - 26
publisher
Wiley-Blackwell
external identifiers
  • wos:000229940600004
  • scopus:20444412371
ISSN
0905-6947
DOI
10.1111/j.1600-0668.2005.00341.x
language
English
LU publication?
yes
id
0dcdfa57-e139-4c4a-b337-e1f3cd399bba (old id 137876)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15910526&dopt=Abstract
date added to LUP
2007-07-10 13:04:05
date last changed
2017-01-01 07:15:32
@article{0dcdfa57-e139-4c4a-b337-e1f3cd399bba,
  abstract     = {We published recently an integrated procedure for applying chemical marker analysis to characterize the microbiology of indoor environments comprising a scheme for extraction and analysis of markers of endotoxin, peptidoglycan/bacterial biomass, and fungal biomass. In the present paper, we report some significant improvements and also new possibilities of the described approach. We found that while 3-hydroxy fatty acids (3-OH FAs) of 10-14 carbon chain lengths are useful endotoxin markers, longer 3-OH FAs (i.e. with 16 carbon atoms and more) may rather serve as markers of Actinobacteria. We introduced C-13-labeled 3-hydroxytridecanoic acid, from labeled Pectinatus cerevisiiphilus, as an internal standard to improve quantification of the 3-OH FAs in the gas chromatography-mass spectrometry analysis. Finally, in experiments aiming to identify a suitable method for collection of house dust for chemical marker analysis, we found that the marker compositions of dusts sedimented on plexiglass plates that were spatially well-distributed in a studied room at different heights above floor level, were undistinguishable. This type of sampling thus appears to be well suited for use, e.g. in epidemiological studies. In summary, the presented work describes important new capabilities of chemical marker analysis in defining human exposure to microorganisms in indoor environments.},
  author       = {Sebastian, Aleksandra and Szponar, Bogumila and Larsson, Lennart},
  issn         = {0905-6947},
  keyword      = {indoor air,muramic acid,chemical markers,microbiology,ergosterol,endotoxin},
  language     = {eng},
  number       = {9},
  pages        = {20--26},
  publisher    = {Wiley-Blackwell},
  series       = {Indoor Air},
  title        = {Characterization of the microbial community in indoor environments by chemical marker analysis: an update and critical evaluation.},
  url          = {http://dx.doi.org/10.1111/j.1600-0668.2005.00341.x},
  volume       = {15 Suppl 9},
  year         = {2005},
}