Advanced

Spectral characteristics of PS II reaction centres: as isolated preparations and when integral to PS II core complexes

Krausz, Elmars; Cox, Nicholas and Peterson Årsköld, Sindra LU (2008) In Photosynthesis Research 98(1-3). p.207-217
Abstract
The discovery that the native PS II enzyme undergoes charge separation via an absorption extending to 730 nm has led us to re-examine the low-temperature absorption spectra of Nanba-Satoh PS II reaction centre preparations with particular focus on the long wavelength region. It is shown that these preparations do not exhibit absorption in the 700-730 nm region at 1.7 K. Absorption in the Nanba-Satoh type preparations analogous to the 'red tail' as observed in functional PS II core complexes is likely shifted to higher energy by >20 nm. Spectral changes associated with the stable reduction of pheo(a) in chemically treated reaction centre preparations are also revisited. Dithionite treatment of PS II preparations in the dark leads to... (More)
The discovery that the native PS II enzyme undergoes charge separation via an absorption extending to 730 nm has led us to re-examine the low-temperature absorption spectra of Nanba-Satoh PS II reaction centre preparations with particular focus on the long wavelength region. It is shown that these preparations do not exhibit absorption in the 700-730 nm region at 1.7 K. Absorption in the Nanba-Satoh type preparations analogous to the 'red tail' as observed in functional PS II core complexes is likely shifted to higher energy by >20 nm. Spectral changes associated with the stable reduction of pheo(a) in chemically treated reaction centre preparations are also revisited. Dithionite treatment of PS II preparations in the dark leads to changes of pigment-pigment and/or pigment protein interactions, as evidenced by changes in absorption and CD spectra. Absorption and CD changes associated with stable Pheo(D1) photo-reduction in PS II core complexes and Nanba-Satoh preparations are compared. For Nanba-Satoh preparations, Q(y) bleaches are similar to 39X broader than in PS II core complexes and are blue-shifted by similar to 4 nm. These data are discussed in terms of current models of PS II, and suggest a need to consider protein-induced changes of some electronic properties of reaction centre pigments. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Charge separation, PS II, Absorption, CD, Spectral assignment
in
Photosynthesis Research
volume
98
issue
1-3
pages
207 - 217
publisher
Springer
external identifiers
  • wos:000261577900019
  • scopus:57849147123
ISSN
0166-8595
DOI
10.1007/s11120-008-9328-8
language
English
LU publication?
yes
id
a7048b0a-df0f-4de0-8275-5874def0b374 (old id 1379150)
date added to LUP
2009-04-24 09:08:50
date last changed
2017-01-01 05:26:23
@article{a7048b0a-df0f-4de0-8275-5874def0b374,
  abstract     = {The discovery that the native PS II enzyme undergoes charge separation via an absorption extending to 730 nm has led us to re-examine the low-temperature absorption spectra of Nanba-Satoh PS II reaction centre preparations with particular focus on the long wavelength region. It is shown that these preparations do not exhibit absorption in the 700-730 nm region at 1.7 K. Absorption in the Nanba-Satoh type preparations analogous to the 'red tail' as observed in functional PS II core complexes is likely shifted to higher energy by >20 nm. Spectral changes associated with the stable reduction of pheo(a) in chemically treated reaction centre preparations are also revisited. Dithionite treatment of PS II preparations in the dark leads to changes of pigment-pigment and/or pigment protein interactions, as evidenced by changes in absorption and CD spectra. Absorption and CD changes associated with stable Pheo(D1) photo-reduction in PS II core complexes and Nanba-Satoh preparations are compared. For Nanba-Satoh preparations, Q(y) bleaches are similar to 39X broader than in PS II core complexes and are blue-shifted by similar to 4 nm. These data are discussed in terms of current models of PS II, and suggest a need to consider protein-induced changes of some electronic properties of reaction centre pigments.},
  author       = {Krausz, Elmars and Cox, Nicholas and Peterson Årsköld, Sindra},
  issn         = {0166-8595},
  keyword      = {Charge separation,PS II,Absorption,CD,Spectral assignment},
  language     = {eng},
  number       = {1-3},
  pages        = {207--217},
  publisher    = {Springer},
  series       = {Photosynthesis Research},
  title        = {Spectral characteristics of PS II reaction centres: as isolated preparations and when integral to PS II core complexes},
  url          = {http://dx.doi.org/10.1007/s11120-008-9328-8},
  volume       = {98},
  year         = {2008},
}