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The role of lipid rafts in LPS-induced signaling in a macrophage cell line.

Olsson, Sandra LU and Sundler, Roger LU (2006) In Molecular Immunology 43(May 16). p.607-612
Abstract
The significance of lipid rafts in lipopolysaccharide (LPS) signaling in macrophages was studied through isolation of them by gradient centrifugation and subsequent visualization of signal molecules using antibodies. LPS signaling is initiated by binding to Toll-like receptor-4 (TLR4) and the co-receptor CD-14, leading to activation of downstream targets, such as MAP kinases. In this study, we show that LPS causes translocation of CD-14 and MAP kinases (ERK-2 and p38) to lipid rafts in the macrophage cell line RAW 264.7. The adaptor proteins MyD88 and Gab-2, on the other hand, were not detected in the lipid raft fractions. These results indicate that lipid rafts play a role in LPS-induced signaling in macrophages. (C) 2005 Elsevier Ltd.... (More)
The significance of lipid rafts in lipopolysaccharide (LPS) signaling in macrophages was studied through isolation of them by gradient centrifugation and subsequent visualization of signal molecules using antibodies. LPS signaling is initiated by binding to Toll-like receptor-4 (TLR4) and the co-receptor CD-14, leading to activation of downstream targets, such as MAP kinases. In this study, we show that LPS causes translocation of CD-14 and MAP kinases (ERK-2 and p38) to lipid rafts in the macrophage cell line RAW 264.7. The adaptor proteins MyD88 and Gab-2, on the other hand, were not detected in the lipid raft fractions. These results indicate that lipid rafts play a role in LPS-induced signaling in macrophages. (C) 2005 Elsevier Ltd. All rights reserved. (Less)
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author
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organization
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type
Contribution to journal
publication status
published
subject
keywords
MAP kinases, ERK, TLR4, CD-14, p38
in
Molecular Immunology
volume
43
issue
May 16
pages
607 - 612
publisher
Pergamon Press Ltd.
external identifiers
  • pmid:15904959
  • wos:000234952300013
  • scopus:28944455001
ISSN
1872-9142
DOI
10.1016/j.molimm.2005.04.011
language
English
LU publication?
yes
id
7addfbf4-c0a9-4c24-b082-728c71f767d0 (old id 137923)
date added to LUP
2016-04-01 17:15:51
date last changed
2021-08-04 01:23:20
@article{7addfbf4-c0a9-4c24-b082-728c71f767d0,
  abstract     = {The significance of lipid rafts in lipopolysaccharide (LPS) signaling in macrophages was studied through isolation of them by gradient centrifugation and subsequent visualization of signal molecules using antibodies. LPS signaling is initiated by binding to Toll-like receptor-4 (TLR4) and the co-receptor CD-14, leading to activation of downstream targets, such as MAP kinases. In this study, we show that LPS causes translocation of CD-14 and MAP kinases (ERK-2 and p38) to lipid rafts in the macrophage cell line RAW 264.7. The adaptor proteins MyD88 and Gab-2, on the other hand, were not detected in the lipid raft fractions. These results indicate that lipid rafts play a role in LPS-induced signaling in macrophages. (C) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Olsson, Sandra and Sundler, Roger},
  issn         = {1872-9142},
  language     = {eng},
  number       = {May 16},
  pages        = {607--612},
  publisher    = {Pergamon Press Ltd.},
  series       = {Molecular Immunology},
  title        = {The role of lipid rafts in LPS-induced signaling in a macrophage cell line.},
  url          = {http://dx.doi.org/10.1016/j.molimm.2005.04.011},
  doi          = {10.1016/j.molimm.2005.04.011},
  volume       = {43},
  year         = {2006},
}