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Methodological improvements for measuring eicosanoids and cytokines in exhaled breath condensate.

Tufvesson, Ellen LU and Bjermer, Leif LU (2006) In Respiratory Medicine 100(1). p.34-38
Abstract
Background: Exhaled breath condensate (EBC) is simple to cottect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentrations of inflammatory markers, potential loss in the sampling system may have great influence. The aim of the present study was to se if evaporation and plastic coating could facilitate detection. Methodology: Through methodological improvements, we have now made it possible to measure EBC concentrations of eicosanoids and cytokines in our system. Due to absorbance of both fatty acid derivates and proteins to several plastics,... (More)
Background: Exhaled breath condensate (EBC) is simple to cottect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentrations of inflammatory markers, potential loss in the sampling system may have great influence. The aim of the present study was to se if evaporation and plastic coating could facilitate detection. Methodology: Through methodological improvements, we have now made it possible to measure EBC concentrations of eicosanoids and cytokines in our system. Due to absorbance of both fatty acid derivates and proteins to several plastics, the first step is coating of all surfaces with bovine serum albumin and Tween 20. Since several assays are sensitive to these factors, the methodology has to be standardised to avoid false results. Secondly, Larger amounts of EBC have to be vacuum-dried, and thereafter resolved in the respective assay buffers. The EBCs have to be concentrated 5-10 times, depending on samples and assay sensitivity. Results: Due to these improvements we can measure, for example, cysteinyl-leukotrienes, leukotriene B-4, prostaglandin E and 8-isoprostane. High sensitivity assays have also made it possible to measure cytokines, for example, interleukin (IL)-1 beta, IL-8 and IL-13. Summary: We are aware of different results from other tabs. However, it seems essential to coat and to concentrate the samples in order to achieve reliable and measurable results. (c) 2005 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
prostaglandin, leukotriene, exhaled breath condensate, asthma, interteukin
in
Respiratory Medicine
volume
100
issue
1
pages
34 - 38
publisher
Elsevier
external identifiers
  • pmid:15894480
  • wos:000234588400006
  • scopus:29844433423
  • pmid:15894480
ISSN
1532-3064
DOI
10.1016/j.rmed.2005.04.007
language
English
LU publication?
yes
id
7c44e60c-5bba-4dfb-9f77-2703e9ecb57b (old id 137996)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=15894480&dopt=Abstract
date added to LUP
2016-04-01 15:26:29
date last changed
2020-12-08 03:36:54
@article{7c44e60c-5bba-4dfb-9f77-2703e9ecb57b,
  abstract     = {Background: Exhaled breath condensate (EBC) is simple to cottect and as such a non-invasive method that has attracted substantial interest in the last few years. However, several methodological concerns have been raised and it has been difficult to reproduce results between different centres. Because of low concentrations of inflammatory markers, potential loss in the sampling system may have great influence. The aim of the present study was to se if evaporation and plastic coating could facilitate detection. Methodology: Through methodological improvements, we have now made it possible to measure EBC concentrations of eicosanoids and cytokines in our system. Due to absorbance of both fatty acid derivates and proteins to several plastics, the first step is coating of all surfaces with bovine serum albumin and Tween 20. Since several assays are sensitive to these factors, the methodology has to be standardised to avoid false results. Secondly, Larger amounts of EBC have to be vacuum-dried, and thereafter resolved in the respective assay buffers. The EBCs have to be concentrated 5-10 times, depending on samples and assay sensitivity. Results: Due to these improvements we can measure, for example, cysteinyl-leukotrienes, leukotriene B-4, prostaglandin E and 8-isoprostane. High sensitivity assays have also made it possible to measure cytokines, for example, interleukin (IL)-1 beta, IL-8 and IL-13. Summary: We are aware of different results from other tabs. However, it seems essential to coat and to concentrate the samples in order to achieve reliable and measurable results. (c) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Tufvesson, Ellen and Bjermer, Leif},
  issn         = {1532-3064},
  language     = {eng},
  number       = {1},
  pages        = {34--38},
  publisher    = {Elsevier},
  series       = {Respiratory Medicine},
  title        = {Methodological improvements for measuring eicosanoids and cytokines in exhaled breath condensate.},
  url          = {http://dx.doi.org/10.1016/j.rmed.2005.04.007},
  doi          = {10.1016/j.rmed.2005.04.007},
  volume       = {100},
  year         = {2006},
}