Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix
(2003) In Chromatographia 58(11-12). p.707-712- Abstract
- Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.
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https://lup.lub.lu.se/record/138354
- author
- Palm, A ; Hakansson, L and Marko-Varga, György LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Chromatographia
- volume
- 58
- issue
- 11-12
- pages
- 707 - 712
- publisher
- Vieweg Verlag
- external identifiers
-
- wos:000224674500005
- scopus:0346690016
- ISSN
- 0009-5893
- DOI
- 10.1365/s10337-003-0104-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 00d11dd2-5989-4c03-b101-d0abf3b29b48 (old id 138354)
- date added to LUP
- 2016-04-01 15:58:10
- date last changed
- 2022-04-22 18:43:12
@article{00d11dd2-5989-4c03-b101-d0abf3b29b48, abstract = {{Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.}}, author = {{Palm, A and Hakansson, L and Marko-Varga, György}}, issn = {{0009-5893}}, language = {{eng}}, number = {{11-12}}, pages = {{707--712}}, publisher = {{Vieweg Verlag}}, series = {{Chromatographia}}, title = {{Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix}}, url = {{http://dx.doi.org/10.1365/s10337-003-0104-9}}, doi = {{10.1365/s10337-003-0104-9}}, volume = {{58}}, year = {{2003}}, }