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Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix

Palm, A; Hakansson, L and Marko-Varga, György LU (2003) In Chromatographia 58(11-12). p.707-712
Abstract
Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chromatographia
volume
58
issue
11-12
pages
707 - 712
publisher
Vieweg Verlag
external identifiers
  • wos:000224674500005
  • scopus:0346690016
ISSN
0009-5893
DOI
10.1365/s10337-003-0104-9
language
English
LU publication?
yes
id
00d11dd2-5989-4c03-b101-d0abf3b29b48 (old id 138354)
date added to LUP
2007-06-27 13:52:55
date last changed
2018-01-07 08:55:42
@article{00d11dd2-5989-4c03-b101-d0abf3b29b48,
  abstract     = {Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.},
  author       = {Palm, A and Hakansson, L and Marko-Varga, György},
  issn         = {0009-5893},
  language     = {eng},
  number       = {11-12},
  pages        = {707--712},
  publisher    = {Vieweg Verlag},
  series       = {Chromatographia},
  title        = {Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix},
  url          = {http://dx.doi.org/10.1365/s10337-003-0104-9},
  volume       = {58},
  year         = {2003},
}