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Amperometric biosensor-based flow-through microdetector for microdialysis applications

Gaspar, Szilveszter LU ; Wang, X W; Suzuki, H and Csöregi, Elisabeth LU (2004) In Analytica Chimica Acta 525(1). p.75-82
Abstract
A new flow-through electrochemical microcell was fabricated, in order to be used as an on-line detector in microdialysis-based investigations. The microcell is obtained by sandwich-like assembling the electrode system patterned on a glass chip and a silicon rubber gasket bearing the fluidic elements (inlet, outlet, flow channel, and containers for reference and counter electrode). Four Pt microelectrodes (100 mum x 100 mum squares), a Pt counter electrode, and a Ag reference electrode were patterned on a 9 mm x 14 mm glass chip by thin film technology. The silicon rubber cover was fabricated using a polydimethylsiloxane based precursor and a plastic mold, and it avoids significant dilution of the sample before getting in contact with the... (More)
A new flow-through electrochemical microcell was fabricated, in order to be used as an on-line detector in microdialysis-based investigations. The microcell is obtained by sandwich-like assembling the electrode system patterned on a glass chip and a silicon rubber gasket bearing the fluidic elements (inlet, outlet, flow channel, and containers for reference and counter electrode). Four Pt microelectrodes (100 mum x 100 mum squares), a Pt counter electrode, and a Ag reference electrode were patterned on a 9 mm x 14 mm glass chip by thin film technology. The silicon rubber cover was fabricated using a polydimethylsiloxane based precursor and a plastic mold, and it avoids significant dilution of the sample before getting in contact with the sensing electrodes. The microdetector can be directly connected to the outlet of the microdialysis probe reducing significantly the delay between sampling and detection. The microelectrodes were modified with enzyme-based chemistries in order to detect glucose, glutamate, and choline by electrooxidation of the hydrogen peroxide produced in the reaction of the analytes with their corresponding oxidases. Problems concerning appropriate linear range, interference elimination, and cross-talk elimination were addressed. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
525
issue
1
pages
75 - 82
publisher
Elsevier
external identifiers
  • wos:000224593600009
  • scopus:4944255262
ISSN
1873-4324
DOI
10.1016/j.aca.2004.07.041
language
English
LU publication?
yes
id
b33b8763-34de-469e-9cdd-a0cd46e6321e (old id 138359)
date added to LUP
2007-07-02 10:34:23
date last changed
2017-01-01 06:43:39
@article{b33b8763-34de-469e-9cdd-a0cd46e6321e,
  abstract     = {A new flow-through electrochemical microcell was fabricated, in order to be used as an on-line detector in microdialysis-based investigations. The microcell is obtained by sandwich-like assembling the electrode system patterned on a glass chip and a silicon rubber gasket bearing the fluidic elements (inlet, outlet, flow channel, and containers for reference and counter electrode). Four Pt microelectrodes (100 mum x 100 mum squares), a Pt counter electrode, and a Ag reference electrode were patterned on a 9 mm x 14 mm glass chip by thin film technology. The silicon rubber cover was fabricated using a polydimethylsiloxane based precursor and a plastic mold, and it avoids significant dilution of the sample before getting in contact with the sensing electrodes. The microdetector can be directly connected to the outlet of the microdialysis probe reducing significantly the delay between sampling and detection. The microelectrodes were modified with enzyme-based chemistries in order to detect glucose, glutamate, and choline by electrooxidation of the hydrogen peroxide produced in the reaction of the analytes with their corresponding oxidases. Problems concerning appropriate linear range, interference elimination, and cross-talk elimination were addressed. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Gaspar, Szilveszter and Wang, X W and Suzuki, H and Csöregi, Elisabeth},
  issn         = {1873-4324},
  language     = {eng},
  number       = {1},
  pages        = {75--82},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Amperometric biosensor-based flow-through microdetector for microdialysis applications},
  url          = {http://dx.doi.org/10.1016/j.aca.2004.07.041},
  volume       = {525},
  year         = {2004},
}