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Evasion of macrophage scavenger receptor A-mediated recognition by pathogenic streptococci

Areschoug, Thomas LU ; Waldemarsson, Johan LU and Gordon, Siamon (2008) In European Journal of Immunology 38(11). p.3068-3079
Abstract
PRR recognize conserved structures on pathogenic microbes and are important for the defense against invading microorganisms. However, accumulating evidence indicates that many pathogens have evolved mechanisms to avoid recognition by PRR. One type of PRR is the macrophage scavenger receptor A (SR-A), which has been shown to play an important role in recognition and non-opsonic phagocytosis of pathogenic bacteria. The bacterial ligands for SR-A have been suggested to be LPS or lipoteichoic acid. Here, we use murine bone marrow-derived macrophages to analyze the role of SR-A in non-opsonic phagocytosis of two major Gram-positive pathogens, streptococcus agalactiae (group B streptococcus; GBS) and Streptococcus pyogenes. We show that the... (More)
PRR recognize conserved structures on pathogenic microbes and are important for the defense against invading microorganisms. However, accumulating evidence indicates that many pathogens have evolved mechanisms to avoid recognition by PRR. One type of PRR is the macrophage scavenger receptor A (SR-A), which has been shown to play an important role in recognition and non-opsonic phagocytosis of pathogenic bacteria. The bacterial ligands for SR-A have been suggested to be LPS or lipoteichoic acid. Here, we use murine bone marrow-derived macrophages to analyze the role of SR-A in non-opsonic phagocytosis of two major Gram-positive pathogens, streptococcus agalactiae (group B streptococcus; GBS) and Streptococcus pyogenes. We show that the polysaccharide capsule of GBS and the surface M protein of S. pyogenes, two important virulence factors, prevent SR-A-mediated non-opsonic phagocytosis of streptococci. The sialic acid moiety of the GBS capsule was crucial for its ability to prevent recognition by SR-A. Moreover, we show that a ligand on GBS recognized by SR-A in the absence of capsule is the surface lipoprotein BIr. These findings represent the first example of a microbial strategy to prevent recognition by SR-A and suggest that bacterial surface proteins may be of importance as ligands for SR-A. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Scavenger receptor A, Polysaccharide capsule, M protein, Macrophage, streptococcus
in
European Journal of Immunology
volume
38
issue
11
pages
3068 - 3079
publisher
John Wiley & Sons Inc.
external identifiers
  • wos:000261458000011
  • scopus:58149337016
ISSN
1521-4141
DOI
10.1002/eji.200838457
language
English
LU publication?
yes
id
4181ac13-ebee-4f8d-a3f2-bdf44f6fd5d0 (old id 1383920)
date added to LUP
2016-04-01 12:10:43
date last changed
2022-04-13 07:11:08
@article{4181ac13-ebee-4f8d-a3f2-bdf44f6fd5d0,
  abstract     = {{PRR recognize conserved structures on pathogenic microbes and are important for the defense against invading microorganisms. However, accumulating evidence indicates that many pathogens have evolved mechanisms to avoid recognition by PRR. One type of PRR is the macrophage scavenger receptor A (SR-A), which has been shown to play an important role in recognition and non-opsonic phagocytosis of pathogenic bacteria. The bacterial ligands for SR-A have been suggested to be LPS or lipoteichoic acid. Here, we use murine bone marrow-derived macrophages to analyze the role of SR-A in non-opsonic phagocytosis of two major Gram-positive pathogens, streptococcus agalactiae (group B streptococcus; GBS) and Streptococcus pyogenes. We show that the polysaccharide capsule of GBS and the surface M protein of S. pyogenes, two important virulence factors, prevent SR-A-mediated non-opsonic phagocytosis of streptococci. The sialic acid moiety of the GBS capsule was crucial for its ability to prevent recognition by SR-A. Moreover, we show that a ligand on GBS recognized by SR-A in the absence of capsule is the surface lipoprotein BIr. These findings represent the first example of a microbial strategy to prevent recognition by SR-A and suggest that bacterial surface proteins may be of importance as ligands for SR-A.}},
  author       = {{Areschoug, Thomas and Waldemarsson, Johan and Gordon, Siamon}},
  issn         = {{1521-4141}},
  keywords     = {{Scavenger receptor A; Polysaccharide capsule; M protein; Macrophage; streptococcus}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{3068--3079}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{European Journal of Immunology}},
  title        = {{Evasion of macrophage scavenger receptor A-mediated recognition by pathogenic streptococci}},
  url          = {{http://dx.doi.org/10.1002/eji.200838457}},
  doi          = {{10.1002/eji.200838457}},
  volume       = {{38}},
  year         = {{2008}},
}