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Biosensor based on cellobiose dehydrogenase for detection of catecholamines

Stoica, Leonard LU ; Lindgren-Sjolander, A; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2004) In Analytical Chemistry 76(16). p.4690-4696
Abstract
A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear... (More)
A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
76
issue
16
pages
4690 - 4696
publisher
The American Chemical Society
external identifiers
  • wos:000223290300011
  • pmid:15307778
  • scopus:4043124610
ISSN
1520-6882
DOI
10.1021/ac049582j
language
English
LU publication?
yes
id
b8634e4d-befa-42aa-b062-2e3ce6d04138 (old id 138430)
date added to LUP
2007-06-28 08:46:13
date last changed
2017-12-10 03:40:56
@article{b8634e4d-befa-42aa-b062-2e3ce6d04138,
  abstract     = {A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones.},
  author       = {Stoica, Leonard and Lindgren-Sjolander, A and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {1520-6882},
  language     = {eng},
  number       = {16},
  pages        = {4690--4696},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Biosensor based on cellobiose dehydrogenase for detection of catecholamines},
  url          = {http://dx.doi.org/10.1021/ac049582j},
  volume       = {76},
  year         = {2004},
}