Biosensor based on cellobiose dehydrogenase for detection of catecholamines
(2004) In Analytical Chemistry 76(16). p.4690-4696- Abstract
- A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear... (More)
- A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138430
- author
- Stoica, Leonard LU ; Lindgren-Sjolander, A ; Ruzgas, Tautgirdas LU and Gorton, Lo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 76
- issue
- 16
- pages
- 4690 - 4696
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000223290300011
- pmid:15307778
- scopus:4043124610
- ISSN
- 1520-6882
- DOI
- 10.1021/ac049582j
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- b8634e4d-befa-42aa-b062-2e3ce6d04138 (old id 138430)
- date added to LUP
- 2016-04-01 11:43:05
- date last changed
- 2022-01-26 17:12:05
@article{b8634e4d-befa-42aa-b062-2e3ce6d04138, abstract = {{A cellobiose dehydrogenase (CDH)-modified graphite electrode was designed for amperometric detection of catecholamines in the flow injection mode, by their recycling between the graphite electrode (+300 mV vs AgAgCl) and the reduced FAD cofactor of adsorbed CDH, resulting in an amplified response signal. The high efficiency of the enzyme-catecholamine reaction leads to a detection limit below 1 nM and a sensitivity of 15.8 A(.)M(-1.)cm(2) 2 (1150 nA/muM) for noradrenaline, with a coverage of less than 2.5 mug of CDH adsorbed on the electrode surface (0.073 cm(2)). Working parameters such as pH, cellobiose concentration, carrier buffer, and applied potential were optimized, using hydroquinone as a model analyte. The sensitivity, linear range, and amplification factor can be modulated by the steady-state concentration of cellobiose in the flow buffer. The response of the sensor decreases only 2% when run continuously for 4 h in the flow injection mode. The response peak maximum is obtained within 6 s at a flow rate of 0.5 mL/min, representing the time of the entire sample segment to pass the electrode. CDH enzymes from Phanerochaete chrysosporium and Sclerotium rolfsii were investigated, providing different characteristics of the sensor, with sensors made with CDH from P. chrysosporium being the better ones.}}, author = {{Stoica, Leonard and Lindgren-Sjolander, A and Ruzgas, Tautgirdas and Gorton, Lo}}, issn = {{1520-6882}}, language = {{eng}}, number = {{16}}, pages = {{4690--4696}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Biosensor based on cellobiose dehydrogenase for detection of catecholamines}}, url = {{http://dx.doi.org/10.1021/ac049582j}}, doi = {{10.1021/ac049582j}}, volume = {{76}}, year = {{2004}}, }