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Use of laccase-modified electrode for amperometric detection of plant flavonoids

Jarosz-Wilkolazka, A; Ruzgas, Tautgirdas LU and Gorton, Lo LU (2004) In Enzyme and Microbial Technology 35(2-3). p.238-241
Abstract
In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more... (More)
In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more phenolic and non-phenolic rings, although with different sensitivity. (C) 2004 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Enzyme and Microbial Technology
volume
35
issue
2-3
pages
238 - 241
publisher
Elsevier
external identifiers
  • wos:000223032200019
  • scopus:3142566342
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2004.04.016
language
English
LU publication?
yes
id
145be56d-9b43-4672-bfe1-b347597a30b7 (old id 138436)
date added to LUP
2007-06-27 08:58:36
date last changed
2017-07-23 03:39:17
@article{145be56d-9b43-4672-bfe1-b347597a30b7,
  abstract     = {In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more phenolic and non-phenolic rings, although with different sensitivity. (C) 2004 Elsevier Inc. All rights reserved.},
  author       = {Jarosz-Wilkolazka, A and Ruzgas, Tautgirdas and Gorton, Lo},
  issn         = {0141-0229},
  language     = {eng},
  number       = {2-3},
  pages        = {238--241},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Use of laccase-modified electrode for amperometric detection of plant flavonoids},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2004.04.016},
  volume       = {35},
  year         = {2004},
}