Use of laccase-modified electrode for amperometric detection of plant flavonoids
(2004) In Enzyme and Microbial Technology 35(2-3). p.238-241- Abstract
- In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more... (More)
- In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more phenolic and non-phenolic rings, although with different sensitivity. (C) 2004 Elsevier Inc. All rights reserved. (Less)
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https://lup.lub.lu.se/record/138436
- author
- Jarosz-Wilkolazka, A ; Ruzgas, Tautgirdas LU and Gorton, Lo LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Enzyme and Microbial Technology
- volume
- 35
- issue
- 2-3
- pages
- 238 - 241
- publisher
- Elsevier
- external identifiers
-
- wos:000223032200019
- scopus:3142566342
- ISSN
- 0141-0229
- DOI
- 10.1016/j.enzmictec.2004.04.016
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 145be56d-9b43-4672-bfe1-b347597a30b7 (old id 138436)
- date added to LUP
- 2016-04-01 11:46:41
- date last changed
- 2022-02-03 04:57:01
@article{145be56d-9b43-4672-bfe1-b347597a30b7,
abstract = {{In this study, laccase from Cerrena unicolor as a highly active enzyme was adsorptively immobilized on the surface of graphite electrode. The electrode inserted into flow-injection system served as a biosensor for detection of polyphenols, including flavonoids. We used catechin hydrate, epicatechin, epicatechin gallate, prodelphinidin, and caffeic acid as a target compounds in determination. The performed investigations demonstrate that laccase from white rot fungus C unicolor can be applied for the construction of biosensors useful for determination of flavonoids and related compounds in flow-injection system. Electrodes modified with laccase yield responses for both simple compounds (caffeic acid) and compounds with three or more phenolic and non-phenolic rings, although with different sensitivity. (C) 2004 Elsevier Inc. All rights reserved.}},
author = {{Jarosz-Wilkolazka, A and Ruzgas, Tautgirdas and Gorton, Lo}},
issn = {{0141-0229}},
language = {{eng}},
number = {{2-3}},
pages = {{238--241}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Use of laccase-modified electrode for amperometric detection of plant flavonoids}},
url = {{http://dx.doi.org/10.1016/j.enzmictec.2004.04.016}},
doi = {{10.1016/j.enzmictec.2004.04.016}},
volume = {{35}},
year = {{2004}},
}