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Development of a laccase-modified electrode for amperometric detection of mono- and diphenols. The influence of enzyme storage method

Jarosz-Wilkolazka, A; Janusz, G; Ruzgas, Tautgirdas LU ; Gorton, Lo LU ; Malarczyk, E and Leonowicz, A (2004) In Analytical Letters 37(8). p.1497-1513
Abstract
Results are reported for biosensors based on two different preparations of the same enzyme, laccase from Cerrena unicolor, one lyophilized and one stored frozen at - 18degreesC, for monitoring phenolic compounds. The enzyme was adsorbed on graphite electrodes and these were used in a flow through wall jet cell connected to a flow injection set-up. The electrodes were used at - 50 mV vs. AgAgCl. The effect of PH, flow rate of the carrier buffer was investigated as well as the operational and storage stability. Biosensors based on the frozen enzyme preparation were shown to be superior for biosensor construction.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Letters
volume
37
issue
8
pages
1497 - 1513
publisher
Taylor & Francis
external identifiers
  • wos:000222755700004
  • scopus:3142703597
ISSN
0003-2719
DOI
10.1081/AL-120037583
language
English
LU publication?
yes
id
555be7d6-c2ac-46dd-a2fa-dc6f2e198eaa (old id 138476)
date added to LUP
2007-06-27 09:00:27
date last changed
2017-11-05 04:27:46
@article{555be7d6-c2ac-46dd-a2fa-dc6f2e198eaa,
  abstract     = {Results are reported for biosensors based on two different preparations of the same enzyme, laccase from Cerrena unicolor, one lyophilized and one stored frozen at - 18degreesC, for monitoring phenolic compounds. The enzyme was adsorbed on graphite electrodes and these were used in a flow through wall jet cell connected to a flow injection set-up. The electrodes were used at - 50 mV vs. AgAgCl. The effect of PH, flow rate of the carrier buffer was investigated as well as the operational and storage stability. Biosensors based on the frozen enzyme preparation were shown to be superior for biosensor construction.},
  author       = {Jarosz-Wilkolazka, A and Janusz, G and Ruzgas, Tautgirdas and Gorton, Lo and Malarczyk, E and Leonowicz, A},
  issn         = {0003-2719},
  language     = {eng},
  number       = {8},
  pages        = {1497--1513},
  publisher    = {Taylor & Francis},
  series       = {Analytical Letters},
  title        = {Development of a laccase-modified electrode for amperometric detection of mono- and diphenols. The influence of enzyme storage method},
  url          = {http://dx.doi.org/10.1081/AL-120037583},
  volume       = {37},
  year         = {2004},
}