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Electrical "wiring" of viable Gluconobacter oxydans cells with a flexible osmium-redox polyelectrolyte

Vostiar, Igor LU ; Ferapontova, Elena LU and Gorton, Lo LU (2004) In Electrochemistry Communications 6(7). p.621-626
Abstract
An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH... (More)
An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH dependencies correspond well with the sensitivity profile for ferricyanide as soluble mediator. Significant stability differences for the substrates were found. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pyrroloquinoline quinone, Mediated electron transfer, Gluconobacter
in
Electrochemistry Communications
volume
6
issue
7
pages
621 - 626
publisher
Elsevier
external identifiers
  • wos:000222726100001
  • scopus:2942652392
ISSN
1388-2481
DOI
10.1016/j.elecom.2004.04.017
language
English
LU publication?
yes
id
76e5624c-1f9d-4145-8072-66b362e80ff6 (old id 138484)
date added to LUP
2007-06-27 16:27:18
date last changed
2017-06-25 03:39:40
@article{76e5624c-1f9d-4145-8072-66b362e80ff6,
  abstract     = {An application of a flexible polyvinylimidazole osmium functionalised polymer for efficient electrical wiring of whole Gluconobacter oxydans cells is described. The performance of the redox polymer as a mediator for intact bacterial cells was tested with glucose, ethanol and glycerol, typical substrates of the periplasmically localised PQQ-dependent membrane bound dehydrogenases. The efficiency of the electron transfer with the osmium redox polymer was compared with that of a soluble mediator (ferricyanide). Some basic bioelectrochemical characteristics, including I-max, K-m(app), inactivation constants and sensitivities were estimated for the mentioned substrates. The sensitivity profile for the tested substrates as well as the pH dependencies correspond well with the sensitivity profile for ferricyanide as soluble mediator. Significant stability differences for the substrates were found. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Vostiar, Igor and Ferapontova, Elena and Gorton, Lo},
  issn         = {1388-2481},
  keyword      = {Pyrroloquinoline quinone,Mediated electron transfer,Gluconobacter},
  language     = {eng},
  number       = {7},
  pages        = {621--626},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Electrical "wiring" of viable Gluconobacter oxydans cells with a flexible osmium-redox polyelectrolyte},
  url          = {http://dx.doi.org/10.1016/j.elecom.2004.04.017},
  volume       = {6},
  year         = {2004},
}