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Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy

Niculescu, Mihaela; Gaspar, Szilveszter LU ; Schulte, Albert; Csöregi, Elisabeth LU and Schuhmann, Wolfgang (2004) In Biosensors & Bioelectronics 19(10). p.1175-1184
Abstract
Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to... (More)
Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to visualize the immobilized enzyme microstructures and their localized biochemical activity was observed with high lateral resolution by detecting the enzymatically consumed substrate using K-4[Fe(CN)(6)] as a free-diffusing electron-transfer mediator. (C) 2003 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme/redox-hydrogel microstructure, Microdispenser, Scanning electrochemical microscopy, SECM
in
Biosensors & Bioelectronics
volume
19
issue
10
pages
1175 - 1184
publisher
Elsevier
external identifiers
  • pmid:15046748
  • wos:000220702600006
  • scopus:1642377016
ISSN
1873-4235
DOI
10.1016/j.bios.2003.11.008
language
English
LU publication?
yes
id
9b2ebaa5-4990-4c69-b470-620faefc58ba (old id 138528)
date added to LUP
2007-07-02 15:10:45
date last changed
2017-01-01 07:07:38
@article{9b2ebaa5-4990-4c69-b470-620faefc58ba,
  abstract     = {Redox hydrogel-based micropatterned complex biosensor architectures, used as sensing chemistries in amperometric ethanol or glucose biosensors, were deposited on gold, graphite or glass. Well-localized immobilization of active hydrogels with variable compositions was achieved by dispensing 100 pl droplets of cocktails containing alcohol or glucose dehydrogenase, redox polymer (PVI(13)dmeOs) and crosslinker (PEGDGE) while moving the target surface relative to the position of the nozzle of a piezo-actuated microdispenser. The resulting structures were microscopic patterns of enzyme-containing lines of a redox hydrogel with a line width of about 100 mum. Scanning electrochemical microscopy (SECM) in the amperometric feedback mode was used to visualize the immobilized enzyme microstructures and their localized biochemical activity was observed with high lateral resolution by detecting the enzymatically consumed substrate using K-4[Fe(CN)(6)] as a free-diffusing electron-transfer mediator. (C) 2003 Elsevier B.V. All rights reserved.},
  author       = {Niculescu, Mihaela and Gaspar, Szilveszter and Schulte, Albert and Csöregi, Elisabeth and Schuhmann, Wolfgang},
  issn         = {1873-4235},
  keyword      = {Enzyme/redox-hydrogel microstructure,Microdispenser,Scanning electrochemical microscopy,SECM},
  language     = {eng},
  number       = {10},
  pages        = {1175--1184},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy},
  url          = {http://dx.doi.org/10.1016/j.bios.2003.11.008},
  volume       = {19},
  year         = {2004},
}